LOCUS       sequence-78034-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; used as 
            template for making expression cassette with multiple gRNA target 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pCBC-MT2T3
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        23..98
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(877..896)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(878..896)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aggggcttcg tgccaccaac gagttttaga gctagaaata gcaagttaaa ataaggctag
       61 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttttttgt ccttctgttt
      121 ttttagtcag tctctttttt cagaagtaca acatcttttt tttgtccttc tgttttttta
      181 gtcagtctct tttttcagaa gtacaacatc ttttttttgt ccttctgttt ttttagtcag
      241 tctctttttt cagaagtaca acatcttttt tttgtccttc tgttttttta gtcagtctct
      301 tttttcagaa gtactctatg atgtatcgtt ctgggaaatg tctgtctgtc tacaacccca
      361 taatctatat ttgcaatcac acatctaata ttctctgtga caagacagcc gaacacgact
      421 tgccttccgc acaatacatc atttcttctt agcttttttt cttcttcttc gttcatacag
      481 tttttttttg tttatcagct tacattttct tgaaccgtag ctttcgtttt cttcttttta
      541 actttccatt cggagttttt gtatcttgtt tcatagtttg tcccaggatt agaatgatta
      601 ggcatcgaac cttcaagaat ttgattgaat aaaacatctt cattcttaag atatgaagat
      661 aatcttcaaa aggcccctgg gaatctgaaa gaagagaagc aggcccattt atatgggaaa
      721 gaacaatagt atttcttata taggcccatt taagttgaaa acaatcttca aaagtcccac
      781 atcgcttaga taagaaaacg aagctgagtt tatatacagc tagagtcgaa gtagtgatat
      841 ctaatacctt tggcctattt ggcctggccc aattcgccct atagtgagtc gtattacaat
      901 tcactgg
//
