LOCUS       sequence-78036-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 
            promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHSN6I01
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(154..830)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(197..217)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga
       61 cgtcgcggtg agttcaggct ttttcatatc tcattgcccc ccggatctgc gaaagctcga
      121 gagagataga tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga
      181 acttccttat atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac
      241 gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt
      301 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc
      361 ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc
      421 tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga
      481 tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat
      541 attcttggag tagacgagag tgtcgtgctc caccatgttc acatcaatcc acttgctttg
      601 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
      661 tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc
      721 atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc
      781 aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt
      841 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca
      901 tgttggcaag ctgctctagc caatacgca
//
