LOCUS       sequence-78038-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 
            promoter), Bar resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pBUN501
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(275..322)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     RBS             402..410
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg
       61 tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg
      121 ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg
      181 gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc ggggaaattc
      241 ggatccccaa tacttgtatg gaggcctgag ctcacttctt cttcttcgcc tgccccgcct
      301 tcttcgtcgc cgctggccgc ttgtcgcccc cgagctgaga caggtcgatg cgcgtctcgt
      361 agaggccggt aatcgactgg tggatgaggg tcgcgtccag gacctcctta gtgcttgtgt
      421 acctcttgcg atcgatagtt gtgtcgaagt acttgaaagc agcaggggcg ccgaggttcg
      481 tcagggtgaa gagatgaatg atattctcag cctgctccct gattggcttg tcgcggtgct
      541 tgttgtacgc ggagaggacc ttatccagat tcgcgtcggc caggatcacg cgcttggaga
      601 actcggaaat ctgctcaatg atctcgtcga ggtaatgctt gtgctgctcg acgaacagct
      661 gcttctgctc gttgtcctcg gggctgccct tgagcttctc gtagtgggag gccaggtaga
      721 ggaagttcac atacttggac ggcagagcca gctcgttccc cttctgcagc tcgccagcgg
      781 aagccagcat ccgcttcctg ccgttctcca gctcgaagag tgagtacttg gggagcttaa
      841 tgatcaggtc cttcttcacc tccttgtagc ccttcgcctc caggaaatcg atcgggttct
      901 tctcgaagct ggagcgctcc ataatcgtga tccccagcag ctccttcacg ctcttgagct
      961 tcttgg
//
