LOCUS       sequence-78041-e         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (OsU3 promoter), Bar resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pBUN411
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(289..336)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     RBS             416..424
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta
       61 tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg
      121 tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa
      181 tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac
      241 gatcggggaa attcggatcc ccaatacttg tatggaggcc tgagctcact tcttcttctt
      301 cgcctgcccc gccttcttcg tcgccgctgg ccgcttgtcg cccccgagct gagacaggtc
      361 gatgcgcgtc tcgtagaggc cggtaatcga ctggtggatg agggtcgcgt ccaggacctc
      421 cttagtgctt gtgtacctct tgcgatcgat agttgtgtcg aagtacttga aagcagcagg
      481 ggcgccgagg ttcgtcaggg tgaagagatg aatgatattc tcagcctgct ccctgattgg
      541 cttgtcgcgg tgcttgttgt acgcggagag gaccttatcc agattcgcgt cggccaggat
      601 cacgcgcttg gagaactcgg aaatctgctc aatgatctcg tcgaggtaat gcttgtgctg
      661 ctcgacgaac agctgcttct gctcgttgtc ctcggggctg cccttgagct tctcgtagtg
      721 ggaggccagg tagaggaagt tcacatactt ggacggcaga gccagctcgt tccccttctg
      781 cagctcgcca gcggaagcca gcatccgctt cctgccgttc tccagctcga agagtgagta
      841 cttggggagc ttaatgatca ggtccttctt cacctccttg tagcccttcg cctccaggaa
      901 atcgatcggg ttcttctcga agctggagcg ctccataatc gtgatcccca gcagctcctt
      961 cacgctcttg agcttct
//
