LOCUS       sequence-78042-e         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 
            promoter), Bar resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pBUN6I11
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(292..318)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(325..345)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(352..537)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             complement(574..594)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(610..630)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(649..696)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     RBS             776..784
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta
       61 tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg
      121 tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa
      181 tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac
      241 gatcggggaa attcggatcc ccaatacttg tatggaggcc tgagctcatg aggcgtagtc
      301 agggacatcg tatgggtagg agccgacctt ccgcttcttc ttaggggacc cgggctcctc
      361 gcccttctcc aggcggagaa taacatccgg cttcgtcagc tggtagccga gggacacgag
      421 gttcttgtag ttctccagca tcacgttccg gtaaacgatc tgctgagcag tgtccaggag
      481 cttccactcc tcccttgtga aatcgacgaa cacgtccttg aacgtcacca gggtgcggct
      541 ccaagcggtg agggacttag cgtccatcga gccgaccttc cgcttcttct ttggtgaccc
      601 gcttgagccg accttccgct tcttctttgg cgaccccgag gacccggact tcttcttctt
      661 cgcctgcccc gccttcttcg tcgccgctgg ccgcttgtcg cccccgagct gagacaggtc
      721 gatgcgcgtc tcgtagaggc cggtaatcga ctggtggatg agggtcgcgt ccaggacctc
      781 cttagtgctt gtgtacctct tgcgatcgat agttgtgtcg aagtacttga aagcagcagg
      841 ggcgccgagg ttcgtcaggg tgaagagatg aatgatattc tcagcctgct ccctgattgg
      901 cttgtcgcgg tgcttgttgt acgcggagag gaccttatcc agattcgcgt cggcc
//
