LOCUS       sequence-78043-e         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 
            promoter), Bar resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pBUN6A11
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(292..318)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(325..345)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(352..501)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(508..528)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(544..564)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(583..630)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     RBS             710..718
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta
       61 tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg
      121 tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa
      181 tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac
      241 gatcggggaa attcggatcc ccaatacttg tatggaggcc tgagctcagg aggcgtagtc
      301 gggcacatcg tatgggtatg agccaacctt ccgcttcttc ttaggcgacc ccagcatatc
      361 gaggtcaaaa tcgtccagag cgtccgaccc gagcatatcg aggtcaaagt cgtcgagcgc
      421 gtcgctcccg agcatatcga ggtcgaagtc gtccagggcg tcggagccca gcatatcgag
      481 gtcgaaatcg tcgagggcgt ccgacccgac cttcctcttc ttcttggggc tgccgctgct
      541 gccgaccttc ctcttcttct tagggctgcc tgatgacccg gacttcttct tcttcgcctg
      601 ccccgccttc ttcgtcgccg ctggccgctt gtcgcccccg agctgagaca ggtcgatgcg
      661 cgtctcgtag aggccggtaa tcgactggtg gatgagggtc gcgtccagga cctccttagt
      721 gcttgtgtac ctcttgcgat cgatagttgt gtcgaagtac ttgaaagcag caggggcgcc
      781 gaggttcgtc agggtgaaga gatgaatgat attctcagcc tgctccctga ttggcttgtc
      841 gcggtgcttg ttgtacgcgg agaggacctt atccagattc gcgtcggcca ggatcacgcg
      901 cttggagaac tcggaaatct gctcaatgat ctcgtcgagg taatgcttgt gctgctcgac
      961 gaacagctgc ttctgctcgt
//
