LOCUS       sequence-78055-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Stennicke HR, Deveraux QL, Humke EW, Reed JC, Dixit VM, Salvesen GS
  TITLE     Caspase-9 can be activated without proteolytic processing.
  JOURNAL   J Biol Chem. 1999 Mar 26. 274(13):8359-62.
  PUBMED    10085063
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Mar 26. 274(13):8359-62."
COMMENT     Sequence Label: pcDNA3-Casp9 C287A
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5..22
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             549..560
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 tagcatttag gtgacactat agaatagggc cctctagatt atgatgtttt aaagaaaagt
       61 tttttccgga ggaaattaaa gcaaccaggc atctgtttat aaatcccttt caccgaaaca
      121 gcattagcga ccctaagcag gagggactgc aggtcttcag agtgagccca ctgctcaaag
      181 atgtcgtcca gggtctcaac gtaccaggag ccactcttgg ggtccctcca ggaaacaaaa
      241 cctgggaaag tagagtagga cacaaagatg tcactgggtg tgggcaaact agatatggcg
      301 tccagctggt cgaaggtcct caaaccttcc tggaacgggg tggcatctgg ctcggggtta
      361 ctgccagggg actcgtcttc aggggaagtg gaggccacct caaacccatg gtctttctgc
      421 tccccaccag cggcctggat gaaaaagagc ttgggcttcc ctcccaggct ggggcagctg
      481 gtcccattga agatgttcac aatcttctcg accgacacag ggcatccatc tgtgccgtag
      541 acagcccctg ggaactgcag gtggctggcc tgacagccgt gagagagaat gaccaccacg
      601 cagcagtcca gagcaccgtg gtcctgctgc gccagctcca gcaaagccag caccattttc
      661 ttggcagtca ggtcgccctt cacctccacc atgaaatgca gcgaggagaa gcgacgccgc
      721 aacttctcac agtcgatgtt ggagccagtg cgggtgcgga gcccggactc acggcagaag
      781 ttcacattgt tgataatgag gcagtggcca cagggctcca tgctcaggat gtaagccaaa
      841 tctgcatttc ccctcaaact ctcaagagca ccgacatcac caaatcctcc agaaccaatg
      901 tccactggtc tgggtgtttc cggtctgaga acctctggtt tgcgaatctc tggtctgagc
      961 accactgggg taaggttttc tagggttggc ttcga
//
