LOCUS       sequence-78064-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Golden Gate entry vector at position 6, LacZ, final entry vector for
            the assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pGGEV_6'_XcmI-LacZ
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          50..622
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     714..731
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    848..869
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(884..889,890..907,908..914)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ctttgccaaa atgatgagac agcacaacaa ccagcacgtt gcccaggagc tgtaggaaag
       61 agaagaaggc atgaacatgg ttagcagagg ggcccggttt ggactcagag tattttatcc
      121 tcatctcaaa cagtgtatat cattgtaacc ataaagagaa aggcaggatg atgaccaggg
      181 tgtagttgtt tctaccaata agaatatttc cacgccagcc agaatttata tgcagaaata
      241 ttctacctta tcatttaatt ataacaattg ttctctaaaa ctgtgctgaa gtacaatata
      301 atataccctg attgccttga aaaaaaagtg attagagaaa gtacttacaa tctgacaaat
      361 aaacaaaagt gaatttaaaa attcgttaca aatgcaagct aaagtttaac gaaaaagtta
      421 cagaaaatga aaagaaaata agaggagaca atggttgtca acagagtaga aagtgaaaga
      481 aacaaaatta tcatgagggt ccatggtgat acaagggaca tcttcccatt ctaaacaaca
      541 ccctgaaaac tttgccccct ccatataaca tgaattttac aatagcgaaa aagaaagaac
      601 aatcaagggt ccccaaactc accctgaagt tctcaggatc ggtcgagatc cttacttagt
      661 tacccgggag gatgagggtc tcacttaggt accagcggtg gcgtggcgag cgcagcgagt
      721 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
      781 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
      841 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
      901 cggctcgtat gttgtgtg
//
