LOCUS       sequence-78066-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for Cre-lox cassette exchange of C-terminal HA-auxin-inducible 
            degron tagged gene sequences together with the S. pombe spo5 gene 
            DSR element into the S. pombe urg1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Watson AT, Daigaku Y, Mohebi S, Etheridge TJ, Chahwan C, Murray JM, 
            Carr AM
  TITLE     Optimisation of the Schizosaccharomyces pombe urg1 Expression 
            System.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83800. doi: 
            10.1371/journal.pone.0083800.
  PUBMED    24376751
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83800. doi"; pages: " 
            10.1371/journal.pone.0083800"
COMMENT     Sequence Label: pAW8ENdeI-cHAIAA7-spo5DSR
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     85..107
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     99..116
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     100..116
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(620..823)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
       61 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
      121 tcataacttc gtatataata ccatatacga agttatcccg gggctttgtc taacaggttt
      181 tatgttggtt taagtttgag tatgtgataa gtgtttgaaa agatttcgat attagagttt
      241 gatgttcaag taaatgctgg aaagatgttt aaatttaatt agcagcagaa gtagcgggat
      301 gggcatgata tggcgtagtc ccgggtcaag ctctgctctt gcacttctcc atcgccctcg
      361 gagcgagacc aatggcatcc gatcctttca tgagacgtaa acgcttgcat gtatcgacga
      421 acattggcca aggaacgtcg ccgacgagca tccaatcacc gtctttgtct tcataagagg
      481 gaacatagtc ccagctattc accaaatcca tcaatttcct ctcattcatg aagtctatca
      541 ttccttcttc tcctccatgt ttgcccatgg taaaagagct gaacatgttg gacaaagcat
      601 tagaaagctc atcgtagctt ttatacatcc tcaaatcgat tttcctcaag tacggtgctc
      661 cgtccattga taccttcacg aacgccgccg cctccgggcc accgcttgat ttttggcagg
      721 aaaccatcac gttcttccgg tatgatctca ccggtggcca tcccacaact tgtgccttgg
      781 ccggaggttt ggctggatct ttaggacaag cactcttctc cttggaatca aaagtcacga
      841 cgtcatgagt cgtagatcct tccttgtttg caggctcatt attcagattt agcttcagat
      901 caaccgtctc tgagaaccct ctcttgtttc
//
