LOCUS       sequence-78076-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:mTurquoise2-Tub1+3'UTR::URA3
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             606..614
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cgctgcaggt cgaccggccc agtttttgaa cacatagttc tttctaaatg ggatagtaga
       61 catggggtga aaaacctgat aatatcggta actttaattt tacgggttct tgttttacct
      121 tctaatgaat tttaagtttt gaaaagcaga aaatcagata ttgaaactat agatctgctt
      181 accttgaagt tcactatttt tggaattctc cttgctaata gttacttcta ctcttgcttt
      241 gtcctggata tcaatggaag atgtcctcat ttccattttt tttttcaatt cgacggcaag
      301 gcgggagaaa aggaactagg gatacaaaaa tgcaattact aatgaaatcc gtgaaataat
      361 catggaaatg ggtgatgtaa gaatctgatg aaacaagtac aaatgtcttt ggcccatcaa
      421 ctacgatgag acttactaag ctttcatcaa atgcaggtta gagaaggaga atggtttttt
      481 tttttatttt ttgtacatag atattagaga aatataataa gtagattata gataagatat
      541 caccaaagag atataacagt agggggaatt ttttttttta caatatactt gaaatataga
      601 aaggataagg aggttggggg cgagagtgaa ccttaaaatt cctcttcctc agcgtatgag
      661 tcggcaccca cttcgatgta atctctttct aaagcagcca aatcttctct agcttcggtg
      721 aattcacctt cttccatacc ttcaccgaca taccagtgga cgaaagcacg tttggcatac
      781 attaaatcga attttctatc gattctcttc caagcctcag caatggatgt ggtatttgac
      841 aacatacaga cggccctatc cacagtggcc aattgtgagt ttggtgtggc agttggaggt
      901 tcgtagcaaa taccgatctt gaaaccagtt gg
//
