LOCUS       sequence-78077-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:CloverGFP-Tub1+3'UTR::URA3
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..33
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        57..272
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 ctagttaact atgcggcatc agagcagatt gtactgagag tgcaccatac caccttttca
       61 attcatcatt ttttttttat tctttttttt gatttcggtt tccttgaaat ttttttgatt
      121 cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga ttggtatata
      181 tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc caactgcaca
      241 gaacaaaaac ctgcaggaaa cgaagataaa tcatgtcgaa agctacatat aaggaacgtg
      301 ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac gaaaagcaaa
      361 caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag ttagttgaag
      421 cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact gatttttcca
      481 tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt ttactcttcg
      541 aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct gcgggtgtat
      601 acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc ccaggtattg
      661 ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc cttttgatgt
      721 tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag ggtactgttg
      781 acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga gacatgggtg
      841 gaagagatga
//
