LOCUS       sequence-78107-e        1109 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NOTE: A new version of this plasmid is now available. See Addgene 
            plasmid 62988..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F
  TITLE     Genome engineering using the CRISPR-Cas9 system.
  JOURNAL   Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. 
            Epub 2013 Oct 24.
  PUBMED    24157548
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.143"; journalName: "Nat Protoc"; date: 
            "2013-11"; volume: "8"; issue: "11"; pages: "2281-308"
COMMENT     Sequence Label: pSpCas9(BB)-2A-Puro (PX459)
FEATURES             Location/Qualifiers
     source          1..1109
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gccttatcca gttcgctcag gccgcctctc tcggccttgg tcagattgtc gaactttctc
       61 tgggtaatca gcttggcgtt cagcagctgc cgccagtagt tcttcatctt cttcacgacc
      121 tcttcggagg gcacgttgtc gctcttgccc cggttcttgt cgcttctggt cagcaccttg
      181 ttgtcgatgg agtcgtcctt cagaaagctc tgaggcacga tatggtccac atcgtagtcg
      241 gacagccggt tgatgtccag ttcctggtcc acgtacatat cccgcccatt ctgcaggtag
      301 tacaggtaca gcttctcgtt ctgcagctgg gtgttttcca cggggtgttc tttcaggatc
      361 tggctgccca gctctttgat gccctcttcg atccgcttca ttctctcgcg gctgttcttc
      421 tgtcccttct gggtggtctg gttctctctg gccatttcga tcacgatgtt ctcgggcttg
      481 tgccggccca tcactttcac gagctcgtcc accaccttca ctgtctgcag gatgcccttc
      541 ttaatggcgg ggctgccggc cagattggca atgtgctcgt gcaggctatc gccctggccg
      601 gacacctggg ctttctggat gtcctcttta aaggtcaggc tgtcgtcgtg gatcagctgc
      661 atgaagtttc tgttggcgaa gccgtcggac ttcaggaaat ccaggattgt cttgccggac
      721 tgcttgtccc ggatgccgtt gatcagcttc cggctcagcc tgccccagcc ggtgtatctc
      781 cgccgcttca gctgcttcat cactttgtcg tcgaacaggt gggcataggt tttcagccgt
      841 tcctcgatca tctctctgtc ctcaaacagt gtcagggtca gcacgatatc ttccagaatg
      901 tcctcgtttt cctcattgtc caggaagtcc ttgtccttga taattttcag cagatcgtgg
      961 tatgtgccca gggaggcgtt gaaccgatct tccacgccgg agatttccac ggagtcgaag
     1021 cactcgattt tcttgaagta gtcctctttc agctgcttca cggtcacttt ccggttggtc
     1081 ttgaacagca ggtccacgat ggccttttt
//
