LOCUS       sequence-78118-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Markus SM, Omer S, Baranowski K, Lee WL
  TITLE     Improved Plasmids for Fluorescent Protein Tagging of Microtubules in
            Saccharomyces cerevisiae.
  JOURNAL   Traffic. 2015 Jul;16(7):773-86. doi: 10.1111/tra.12276. Epub 2015 
            Apr 28.
  PUBMED    25711127
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12276";
            journalName: "Traffic"; date: "2015-07"; volume: "16"; issue: "7"; 
            pages: "773-86"
COMMENT     Sequence Label: pHIS3p:Venus-Tub1+3'UTR::LEU2
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             605..613
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gctgcaggtc gaccggccca gtttttgaac acatagttct ttctaaatgg gatagtagac
       61 atggggtgaa aaacctgata atatcggtaa ctttaatttt acgggttctt gttttacctt
      121 ctaatgaatt ttaagttttg aaaagcagaa aatcagatat tgaaactata gatctgctta
      181 ccttgaagtt cactattttt ggaattctcc ttgctaatag ttacttctac tcttgctttg
      241 tcctggatat caatggaaga tgtcctcatt tccatttttt ttttcaattc gacggcaagg
      301 cgggagaaaa ggaactaggg atacaaaaat gcaattacta atgaaatccg tgaaataatc
      361 atggaaatgg gtgatgtaag aatctgatga aacaagtaca aatgtctttg gcccatcaac
      421 tacgatgaga cttactaagc tttcatcaaa tgcaggttag agaaggagaa tggttttttt
      481 ttttattttt tgtacataga tattagagaa atataataag tagattatag ataagatatc
      541 accaaagaga tataacagta gggggaattt ttttttttac aatatacttg aaatatagaa
      601 aggataagga ggttgggggc gagagtgaac cttaaaattc ctcttcctca gcgtatgagt
      661 cggcacccac ttcgatgtaa tctctttcta aagcagccaa atcttctcta gcttcggtga
      721 attcaccttc ttccatacct tcaccgacat accagtggac gaaagcacgt ttggcataca
      781 ttaaatcgaa ttttctatcg attctcttcc aagcctcagc aatggatgtg gtatttgaca
      841 acatacagac gg
//
