LOCUS       sequence-78122-e         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Komuves LG, Ma XK, Stelnicki E, Rozenfeld S, Oda Y, Largman C
  TITLE     HOXB13 homeodomain protein is cytoplasmic throughout fetal skin 
            development.
  JOURNAL   Dev Dyn 2003 Jun;227(2):192-202.
  PUBMED    12761847
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn"; 
            date: "2003-06"; volume: "227(2)"; pages: "192-202"
COMMENT     Sequence Label: HOXC13(human)-pET
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    75..99
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             114..136
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             155..172
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             182..199
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             203..235
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaattaata
       61 cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt
      121 aactttaaga aggagatata ccatgggcag cagccatcat catcatcatc acagcagcgg
      181 cctggtgccg cgcggcagcc atatggctag catgactggt ggacagcaaa tgggtcgcgg
      241 atccgaattc atgacgactt cgctgctcct gcatccacgc tggccggaga gccttatgta
      301 cgtctatgag gacagcgcgg cggagagcgg catcggcggc ggcggcggag gaggaggcgg
      361 cggcacgggc ggagcggggg gtggctgcag cggagcgagc cccggcaaag ccccgagcat
      421 ggatggtctg ggcagcagct gtccggccag ccactgccgc gacctgcttc cgcaccccgt
      481 gctgggccgc ccaccggctc ccctgggcgc ccctcagggc gccgtctata cggacatccc
      541 ggccccggag gcggcgcgcc agtgtgcccc gccgcccgca ccccccacct cgtccagcgc
      601 caccctgggc tacggctacc cctttggggg cagctactac ggctgccgcc tgtcgcacaa
      661 cgtgaacctg cagcagaagc cttgcgccta ccacccgggc gataaatacc cggagccgtc
//
