LOCUS       sequence-78123-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for Golden Gate assembly; middle entry vector for
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Kirchmaier S, Lust K, Wittbrodt J
  TITLE     Golden GATEway cloning--a combinatorial approach to generate fusion 
            and recombination constructs.
  JOURNAL   PLoS One. 2013 Oct 7;8(10):e76117. doi: 
            10.1371/journal.pone.0076117.
  PUBMED    24116091
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-7"; volume: "8(10):e76117. doi"; pages: " 
            10.1371/journal.pone.0076117"
COMMENT     Sequence Label: pME_GGWDest-
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..23
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      117..126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      121..130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(161..179)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(186..202)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(195..217)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        complement(397..415)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(425..441)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(443..542)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(566..584)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 agctatttag gtgacactat agaatactca agctatgcat ccaacgcgtt gggagctctc
       61 ccatatggtc gacctgcagg cggccgcgaa ttcactagtg atatcgaatt cccgcggccg
      121 ccatggcggc cgggagcatg cgacgtcggg cccaattcgc cctatagtga gtcgtattac
      181 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      241 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      301 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg
      361 cattaagcgc gggtctcgat acggtaccca attcgcccta tagtgagtcg tattacgcgc
      421 gctcactggc cgtcgtttta caagcctgct tttttgtaca aagttggcat tataaaaaag
      481 cattgctcat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
      541 tggggcccga gcttaagact ggccgtctat agtgtcacct aaatcaagct tgctccgacg
      601 tccccaggca gaatggcggt tccctaaacg agcattgctt atatagacct cccattaggc
      661 acgcctaccg cccatttacg tcaatggaac gcccatttgc gtcattgccc ctccccattg
      721 acgtcaatgg ggatgtactt ggcagccatc gcgggccatt taccgccatt gacgtcaatg
      781 ggagtactgc caatgtaccc tggcgtactt ccaatagtaa tgtacttgcc aagttactat
      841 taatagatat tgatgtactg ccaagtgggc catttaccgt cattgacgtc aataggg
//
