LOCUS       sequence-78132-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH41531
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(87..89,90..92,93..806)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(132..153)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(393..412)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     740..761
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
       61 attcattaat cactctgtgg tctcaaatgg tgagcaaggg cgaggagctg ttcaccgggg
      121 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      181 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      241 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cagctacggc gtgcagtgct
      301 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      361 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      421 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      481 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      541 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
      601 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      661 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
      721 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      781 acggcatgga cgagctgtac aagtaagctt tgagaccacg aagtggctct
//
