LOCUS       sequence-78133-e         120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 120)
  AUTHORS   Guo X, Zhang T, Hu Z, Zhang Y, Shi Z, Wang Q, Cui Y, Wang F, Zhao H,
            Chen Y
  TITLE     Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis.
  JOURNAL   Development. 2014 Jan 8.
  PUBMED    24401372
REFERENCE   2  (bases 1 to 120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2014 Jan 8."
COMMENT     Sequence Label: pUC57-Simple-gRNA backbone
FEATURES             Location/Qualifiers
     source          1..120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_RNA        38..113
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 taatacgact cactataggt cgtcttcgag aagacctgtt ttagagctag aaatagcaag
       61 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttaaa
//
