LOCUS       sequence-78140-e         513 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of luciferase reporter mRNA in S2 cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Fukaya T, Tomari Y
  TITLE     MicroRNAs mediate gene silencing via multiple different pathways in 
            drosophila.
  JOURNAL   Mol Cell. 2012 Dec 28;48(6):825-36. doi: 
            10.1016/j.molcel.2012.09.024. Epub 2012 Nov 1.
  PUBMED    23123195
REFERENCE   2  (bases 1 to 513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2012.09.024"; journalName: "Mol Cell"; date: 
            "2012-12-28- 28"; volume: "48"; issue: "6"; pages: "825-36"
COMMENT     Sequence Label: pAWH-Rluc-let-7-A114-N40-HhR
FEATURES             Location/Qualifiers
     source          1..513
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(130..219)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     regulatory      249..258
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    266..290
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(307..358)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
ORIGIN
        1 attttatatt taccttagag ctttaaatct ctgtaggtag tttgtccaat tatgtcacac
       61 cacagaagta aggttccttc acaaagatcc tgctagctta cgtcacgtgg accggtgtcc
      121 gccatgagca gcgtaatctg gaacgtcata tggataggat cctgcatagt ccgggacgtc
      181 atagggatag cccgcatagt caggaacatc gtatgggtaa aagatgcggc ctccaccgcg
      241 gtggagatcc atggtggcgg agctcaccac tttgtacaag aaagctgggt cggcgcgccc
      301 acccttcctg tttcgtcctc acggactcat cagaccggaa aacacatccg gtgacaggaa
      361 gcttaaaccg agaggagaca gaccaggaaa ccagatgcat tttttttttt tttttttttt
      421 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      481 tttttttttt tttttttttt tttttttttt ttt
//
