LOCUS       sequence-78147-e         292 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template DNA for in vitro transcription using T7 polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Fukaya T, Tomari Y
  TITLE     PABP is not essential for microRNA-mediated translational repression
            and deadenylation in vitro.
  JOURNAL   EMBO J. 2011 Nov 25;30(24):4998-5009. doi: 10.1038/emboj.2011.426.
  PUBMED    22117217
REFERENCE   2  (bases 1 to 292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2011"; journalName: "EMBO J"; date: "2011-11-25- 25";
            volume: "30"; issue: "24"; pages: "4998-5009"
COMMENT     Sequence Label: pUC57-Rluc-BoxB-A114-N40
FEATURES             Location/Qualifiers
     source          1..292
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    30..48
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    72..90
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    96..114
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    154..172
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    178..196
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
ORIGIN
        1 taagtccaac taccaaactg gggattcctg ggccctgaag aagggcccct cgactagtcc
       61 aaatacaaac tgggccctga agaagggccc atatagggcc ctgaagaagg gccctatcga
      121 ggatattatc tcgactaagt ccaactacaa actgggccct gaagaagggc ccatataggg
      181 ccctgaagaa gggccctatc gaggatatta tgcggccgct ggccgcctgc agaaaaaaaa
      241 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa
//
