LOCUS       sequence-78162-e         619 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Kawaoka S, Hayashi N, Suzuki Y, Abe H, Sugano S, Tomari Y, Shimada 
            T, Katsuma S
  TITLE     The Bombyx ovary-derived cell line endogenously expresses 
            PIWI/PIWI-interacting RNA complexes.
  JOURNAL   RNA. 2009 Jul;15(7):1258-64. doi: 10.1261/rna.1452209. Epub 2009 May
            21.
  PUBMED    19460866
REFERENCE   2  (bases 1 to 619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1452209"; journalName: "RNA"; date: "2009-07"; volume: 
            "15"; issue: "7"; pages: "1258-64"
COMMENT     Sequence Label: pIZ-Flag6His-Siwi
FEATURES             Location/Qualifiers
     source          1..619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             573..596
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             597..614
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 acaatgtatg gtgctaatgt tgcttcaaca acaattctgt tgaactgtgt tttcatgttt
       61 gccaacaagc acctttatac tcggtggcct ccccaccacc aacttttttg cactgcaaaa
      121 aaacacgctt ttgcacgcgg gcccatacat agtacaaact ctacgtttcg tagactattt
      181 tacataaata gtctacaccg ttgtatacgc tccaaataca ctaccacaca ttgaaccttt
      241 ttgcagtgca aaaaagtacg tgtcggcagt cacgtaggcc ggccttatcg ggtcgcgtcc
      301 tgtcacgtac gaatcacatt atcggaccgg acgagtgttg tcttatcgtg acaggacgcc
      361 agcttcctgt gttgctaacc gcagccggac gcaactcctt atcggaacag gacgcgcctc
      421 catatcagcc gcgcgttatc tcatgcgcgt gaccggacac gaggcgcccg tcccgcttat
      481 cgcgcctata aatacagccc gcaacgatct ggtaaacaca gttgaacagc atctgttcga
      541 atttaaagct tggtaccgag ctcggatcca tggattacaa ggatgacgac gataagcatc
      601 atcatcatca tcatatgtc
//
