LOCUS       sequence-78163-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  baculovirus-based delivery system that enables the efficient 
            incorporation of unnatural amino acids into proteins in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Chatterjee A, Xiao H, Bollong M, Ai HW, Schultz PG
  TITLE     Efficient viral delivery system for unnatural amino acid mutagenesis
            in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. doi: 
            10.1073/pnas.1309584110. Epub 2013 Jul 1.
  PUBMED    23818609
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1309584110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-07-16- 16"; volume: "110"; issue: "29"; pages: "11803-8"
COMMENT     Sequence Label: pAcBac1.tR4-MbPyl
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             113..145
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     polyA_signal    294..428
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(331..350)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     385..404
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        461..709
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     461..481
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     632..651
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        786..880
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     797..816
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    834..852
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    862..880
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ctctattgcc tcttttttct ttatcatagg gttaatcatt ggactattct tggttctccg
       61 agttggtatc catctttgca ttaaattaaa gcacaccaag aaaagacaga tttatacaga
      121 catagagatg aaccgacttg gaaagtgata aggccaggcc ggccaagctt gtcgagaagt
      181 actagaggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
      241 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
      301 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
      361 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
      421 tctggatctg atcactgctt gagcctaggt cgggcaggaa gagggcctat ttcccatgat
      481 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac
      541 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      601 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
      661 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg gaaacctgat
      721 catgtagatc gaacggactc taaatccgtt cagccgggtt agattcccgg ggtttccgtt
      781 ttttaatatt tgcatgtcgc tatgtgttct gggaaatcac cataaacgtg aaatccctat
      841 cagtgataga gacttataag ttccctatca gtgatagaga caccgggg
//
