LOCUS       sequence-7817-ex        9448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9448)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 9448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG425GPD-ccdB
FEATURES             Location/Qualifiers
     source          1..9448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      69..1411
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    401..448
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1438..1542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1543..1611,1612..2403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1761..1780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2574..3162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3063..3082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3316..3333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(3486..3491,3492..3509,3510..3516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3524..3540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3529..3551
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3548..3564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3548..3564
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3583..3603
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3585..3603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3632..4276
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     4170..4193
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     4282..4298
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4282..4298
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    4306..4430
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4455..4460,4461..4478,4479..4485)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4539..5198
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4605..4624)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5543..5848
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5727..5746
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5889..6013)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6046..6062)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6047..6063)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      6065..6312
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6081..6099)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(6330..6349)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6331..6349)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6359..6376)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6359..6375)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6368..6390)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6520..6975
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6607..6626)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6817..6838
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7275..7679
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             7692..8786
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(9279..9298)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9398..9420
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
      121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
      181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
      241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
      301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
      361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
      421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
      481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
      541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
      601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
      661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
      721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
      781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
      841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
      901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
      961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
     1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
     1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
     1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
     1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
     1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
     1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
     1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
     1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2701 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt acctcactca ttaggcaccc
     3481 caggctttac actttatgct tccggctcct atgttgtgtg gaattgtgag cggataacaa
     3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     3601 agggaacaaa agctggagct cttcgagttt atcattatca atactcgcca tttcaaagaa
     3661 tacgtaaata attaatagta gtgattttcc taactttatt tagtcaaaaa attagccttt
     3721 taattctgct gtaacccgta catgcccaaa atagggggcg ggttacacag aatatataac
     3781 accgaaggtg cctgggtgaa caggtttatt cctggcatcc actaaatata atggagcccg
     3841 ctttttaagc tggcatccag aaaaaaaaag aatcccagca ccaaaatatt gttttcttca
     3901 ccaaccatca gttcataggt ccattctctt agcgcaacta cagagaacag gggcacaaac
     3961 aggcaaaaaa cgggcacaac ctcaatggag tgatgcaacc tgcctggagt aaatgatgac
     4021 acaaggcaat tgacccacgc atgtatctat ctcattttct tacaccttct attaccttct
     4081 gctctctctg atttggaaaa agctgaaaaa aaaggttgaa accagttccc tgaaattatt
     4141 cccctacttg actaataagt atataaagac ggtaggtatt gattgtaatt ctgtaaatct
     4201 atttcttaaa cttcttaaat tctactttta tagttagtct tttttttagt tttaaaacac
     4261 cagaacttag tttcgacgga ttctagaact agtggatccc ccatcacaag tttgtacaaa
     4321 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
     4381 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcat
     4441 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
     4501 aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     4561 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     4621 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     4681 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     4741 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     4801 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     4861 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     4921 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     4981 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     5041 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     5101 cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat
     5161 tacaacagta ctgcgatgag tggcagggcg gggcgtaaac gccgcgtgga tccggcttac
     5221 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     5281 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
     5341 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     5401 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     5461 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     5521 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     5581 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     5641 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     5701 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     5761 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     5821 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
     5881 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
     5941 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
     6001 tgtacaaagt ggtgatgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct
     6061 cgagtcatgt aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta
     6121 accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt
     6181 atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc
     6241 gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag
     6301 gctttaattt gcggccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     6361 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     6421 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     6481 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat
     6541 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6601 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6661 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6721 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6781 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6841 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6901 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     6961 taacgtttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     7021 ccgcatatcg acggtcgagg agaacttcta gtatatccac atacctaata ttattgcctt
     7081 attaaaaatg gaatcccaac aattacatca aaatccacat tctcttcaaa atcaattgtc
     7141 ctgtacttcc ttgttcatgt gtgttcaaaa acgttatatt tataggataa ttatactcta
     7201 tttctcaaca agtaattggt tgtttggccg agcggtctaa ggcgcctgat tcaagaaata
     7261 tcttgaccgc agttaactgt gggaatactc aggtatcgta agatgcaaga gttcgaatct
     7321 cttagcaacc attatttttt tcctcaacat aacgagaaca cacaggggcg ctatcgcaca
     7381 gaatcaaatt cgatgactgg aaattttttg ttaatttcag aggtcgcctg acgcatatac
     7441 ctttttcaac tgaaaaattg ggagaaaaag gaaaggtgag aggccggaac cggcttttca
     7501 tatagaatag agaagcgttc atgactaaat gcttgcatca caatacttga agttgacaat
     7561 attatttaag gacctattgt tttttccaat aggtggttag caatcgtctt actttctaac
     7621 ttttcttacc ttttacattt cagcaatata tatatatatt tcaaggatat accattctaa
     7681 tgtctgcccc tatgtctgcc cctaagaaga tcgtcgtttt gccaggtgac cacgttggtc
     7741 aagaaatcac agccgaagcc attaaggttc ttaaagctat ttctgatgtt cgttccaatg
     7801 tcaagttcga tttcgaaaat catttaattg gtggtgctgc tatcgatgct acaggtgtcc
     7861 cacttccaga tgaggcgctg gaagcctcca agaaggttga tgccgttttg ttaggtgctg
     7921 tggctggtcc taaatggggt accggtagtg ttagacctga acaaggttta ctaaaaatcc
     7981 gtaaagaact tcaattgtac gccaacttaa gaccatgtaa ctttgcatcc gactctcttt
     8041 tagacttatc tccaatcaag ccacaatttg ctaaaggtac tgacttcgtt gttgtcagag
     8101 aattagtggg aggtatttac tttggtaaga gaaaggaaga cgatggtgat ggtgtcgctt
     8161 gggatagtga acaatacacc gttccagaag tgcaaagaat cacaagaatg gccgctttca
     8221 tggccctaca acatgagcca ccattgccta tttggtcctt ggataaagct aatcttttgg
     8281 cctcttcaag attatggaga aaaactgtgg aggaaaccat caagaacgaa ttccctacat
     8341 tgaaggttca acatcaattg attgattctg ccgccatgat cctagttaag aacccaaccc
     8401 acctaaatgg tattataatc accagcaaca tgtttggtga tatcatctcc gatgaagcct
     8461 ccgttatccc aggttccttg ggtttgttgc catctgcgtc cttggcctct ttgccagaca
     8521 agaacaccgc atttggtttg tacgaaccat gccacggttc tgctccagat ttgccaaaga
     8581 ataaggttga ccctatcgcc actatcttgt ctgctgcaat gatgttgaaa ttgtcattga
     8641 acttgcctga agaaggtaag gccattgaag atgcagttaa aaaggttttg gatgcaggta
     8701 tcagaactgg tgatttaggt ggttccaaca gtaccaccga agtcggtgat gctgtcgccg
     8761 aagaagttaa gaaaatcctt gcttaaaaag attctctttt tttatgatat ttgtacataa
     8821 actttataaa tgaaattcat aatagaaacg acacgaaatt acaaaatgga atatgttcat
     8881 agggtagacg aaactatata cgcaatctac atacatttat caagaaggag aaaaaggagg
     8941 atagtaaagg aatacaggta agcaaattga tactaatggc tcaacgtgat aaggaaaaag
     9001 aattgcactt taacattaat attgacaagg aggagggcac cacacaaaaa gttaggtgta
     9061 acagaaaatc atgaaactac gattcctaat ttgatattgg aggattttct ctaaaaaaaa
     9121 aaaaatacaa caaataaaaa acactcaatg acctgaccat ttgatggagt ttaagtcaat
     9181 accttcttga agcatttccc ataatggtga aagttccctc aagaatttta ctctgtcaga
     9241 aacggcctta cgacgtagtc gatatggtgc actctcagta caatctgctc tgatgccgca
     9301 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     9361 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     9421 ttttcaccgt catcaccgaa acgcgcga
//
