LOCUS       sequence-78189-e        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid separately encodes a human codon-optimized SpCas9 
            nickase, a tracrRNA and customizable crRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, 
            Jiang W, Marraffini LA, Zhang F
  TITLE     Multiplex Genome Engineering Using CRISPR/Cas Systems.
  JOURNAL   Science. 2013 Jan 3.
  PUBMED    23287718
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Jan 3."
COMMENT     Sequence Label: 
            pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(20..40)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggaattctta gctggcctcc acctttctct tcttcttggg gctgtcgcct cccagctgag
       61 acaggtcgat ccgtgtctcg tacaggccgg tgatgctctg gtggatcagg gtggcgtcca
      121 gcacctcttt ggtgctggtg tacctcttcc ggtcgatggt ggtgtcaaag tacttgaagg
      181 cggcaggggc tcccagattg gtcagggtaa acaggtggat gatattctcg gcctgctctc
      241 tgatgggctt atcccggtgc ttgttgtagg cggacagcac tttgtccaga ttagcgtcgg
      301 ccaggatcac tctcttggag aactcgctga tctgctcgat gatctcgtcc aggtagtgct
      361 tgtgctgttc cacaaacagc tgtttctgct cattatcctc gggggagccc ttcagcttct
      421 catagtggct ggccaggtac aggaagttca catatttgga gggcagggcc agttcgtttc
      481 ccttctgcag ttcgccggca gaggccagca ttctcttccg gccgttttcc agctcgaaca
      541 gggagtactt aggcagcttg atgatcaggt cctttttcac ttctttgtag cccttggctt
      601 ccagaaagtc gatgggattc ttctcgaagc tgcttctttc catgatggtg atccccagca
      661 gctctttcac actcttcagt ttcttggact tgcccttttc cactttggcc accaccagca
      721 cagaataggc cacggtgggg ctgtcgaagc cgccgtactt cttagggtcc cagtccttct
      781 ttctggcgat cagcttatcg ctgttcctct tgggcaggat agactctttg ctgaagccgc
      841 ctgtctgcac ctcggtcttt ttcacgatat tcacttgggg catgctcagc actttccgca
      901 cggtggcaaa atcccggccc ttatcccaca cgatctcccc ggtttcgccg tttgtctcga
      961 tcagaggccg cttccggatc tcgccgttgg ccagggtaat ctcggtcttg aaaaagttca
     1021 tgatgttgct gtagaagaag tacttggcgg tagccttgcc
//
