LOCUS       sequence-78196-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-regulatable dCas9 lentiviral expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Kearns NA, Genga RM, Enuameh MS, Garber M, Wolfe SA, Maehr R
  TITLE     Cas9 effector-mediated regulation of transcription and 
            differentiation in human pluripotent stem cells.
  JOURNAL   Development. 2014 Jan;141(1):219-23. doi: 10.1242/dev.103341.
  PUBMED    24346702
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-01"; volume: "141"; issue: 
            "1"; pages: "219-23"
COMMENT     Sequence Label: pHAGE TRE dCas9
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(184..210)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    263..287
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(337..426)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             complement(427..447)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aggatcagga cgctcgctgc gcccttcgtc tgacgtggca gcgctcgccg tgaggagggg
       61 ggcgcccgcg ggaggcgcca aaacccggcg cggaggccag atcttgggtg ggttactcca
      121 gactgccttg ggaaaagcgc ctcccctacc cggtagaatt tctagtttaa ttaatcatta
      181 ctaagcgtag tctgggacgt cgtatgggta ttcgaaccgc gggccctcta gactcgagcg
      241 gccgccactg tgctggatat caaccacttt gtacaagaaa gctgggtcta gatatctcga
      301 ggcggccgct tatccggatt cgaatcatta ggatccagcg taatctggaa cgtcataagg
      361 atacgatcct gcatagtccg ggacgtcata gggatagccc gcatagtcag gaacatcgta
      421 tgggtatacc ttcctcttct tcttgggccc gccggtgccg tctccaccga gctgagagag
      481 gtcgattctt gtttcataga gccccgtaat tgactgatga atcagtgtgg cgtccaggac
      541 ctcctttgta gaggtgtacc gctttctgtc tatggtggtg tcgaagtact tgaaggctgc
      601 aggcgcgccc aagttggtca gagtaaacaa gtggataatg ttttctgcct gctccctgat
      661 gggcttatcc ctgtgcttat tgtaagcaga aagcacctta tcgaggttag cgtcggcgag
      721 gatcactctt ttggagaact cgcttatttg ctcgatgatc tcatcaaggt agtgtttgtg
      781 ttgttccacg aacagctgct tctgctcatt atcttcggga gaccctttga gcttttcata
      841 gtggctggcc agatacaaga aattaacgta tttagagggc agtgccagct cgttaccttt
      901 ctgcagctcg cccgcactag cgagcattcg tttccgg
//
