LOCUS       sequence-78268-e        9400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Lee Y, Hur I, Park SY, Kim YK, Suh MR, Kim VN
  TITLE     The role of PACT in the RNA silencing pathway.
  JOURNAL   EMBO J. 2006 Feb 8;25(3):522-32. Epub 2006 Jan 19.
  PUBMED    16424907
REFERENCE   2  (bases 1 to 9400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2006-02-8"; volume: "25(3)"; pages: "522-32. Epub 2006 Jan 
            19"
COMMENT     Sequence Label: pcDNA3.1-FLAG-Dicer-delHelicC
FEATURES             Location/Qualifiers
     source          1..9400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             909..932
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4994..5011)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5000..5224
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5270..5698
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5357..5376)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5567..5588
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5707..5727)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5712..6041
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5892..6027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5954..5973
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6108..6902
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6162..6181)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6772..6791
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7076..7197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7113..7132)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7167..7186
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7246..7262)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7246..7262)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7259..7281)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7270..7286
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7294..7300,7301..7318,7319..7324))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7339..7360
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7477..7494)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7648..8233)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7728..7747)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8404..9195,9196..9264))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9027..9046
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9265..9369)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 ccaccatgga ctacaaggac gacgatgaca agtaagctta tcttgagaaa caagtgttcc
      961 aagtcggttg atactggtga gactgacatt gatcctgtca tggatgatga tgacgttttc
     1021 ccaccatatg tgttgaggcc tgacgatggt ggtccacgag tcacaatcaa cacggccatt
     1081 ggacacatca atagatactg tgctagatta ccaagtgatc cgtttactca tctagctcct
     1141 aaatgcagaa cccgagagtt gcctgatggt acattttatt caactcttta tctgccaatt
     1201 aactcacctc ttcgagcctc cattgttggt ccaccaatga gctgtgtacg attggctgaa
     1261 agagttgtag ctctcatttg ctgtgagaaa ctgcacaaaa ttggcgaact ggatgaccat
     1321 ttgatgccag ttgggaaaga gactgttaaa tatgaagagg agcttgattt gcatgatgaa
     1381 gaagagacca gtgttccagg aagaccaggt tccacgaaac gaaggcagtg ctacccaaaa
     1441 gcaattccag agtgtttgag ggatagttat cccagacctg atcagccctg ttacctgtat
     1501 gtgataggaa tggttttaac tacaccttta cctgatgaac tcaactttag aaggcggaag
     1561 ctctatcctc ctgaagatac cacaagatgc tttggaatac tgacggccaa acccatacct
     1621 cagattccac actttcctgt gtacacacgc tctggagagg ttaccatatc cattgagttg
     1681 aagaagtctg gtttcatgtt gtctctacaa atgcttgagt tgattacaag acttcaccag
     1741 tatatattct cacatattct tcggcttgaa aaacctgcac tagaatttaa acctacagac
     1801 gctgattcag catactgtgt tctacctctt aatgttgtta atgactccag cactttggat
     1861 attgacttta aattcatgga agatattgag aagtctgaag ctcgcatagg cattcccagt
     1921 acaaagtata caaaagaaac accctttgtt tttaaattag aagattacca agatgccgtt
     1981 atcattccaa gatatcgcaa ttttgatcag cctcatcgat tttatgtagc tgatgtgtac
     2041 actgatctta ccccactcag taaatttcct tcccctgagt atgaaacttt tgcagaatat
     2101 tataaaacaa agtacaacct tgacctaacc aatctcaacc agccactgct ggatgtggac
     2161 cacacatctt caagacttaa tcttttgaca cctcgacatt tgaatcagaa ggggaaagcg
     2221 cttcctttaa gcagtgctga gaagaggaaa gccaaatggg aaagtctgca gaataaacag
     2281 atactggttc cagaactctg tgctatacat ccaattccag catcactgtg gagaaaagct
     2341 gtttgtctcc ccagcatact ttatcgcctt cactgccttt tgactgcaga ggagctaaga
     2401 gcccagactg ccagcgatgc tggcgtggga gtcagatcac ttcctgcgga ttttagatac
     2461 cctaacttag acttcgggtg gaaaaaatct attgacagca aatctttcat ctcaatttct
     2521 aactcctctt cagctgaaaa tgataattac tgtaagcaca gcacaattgt ccctgaaaat
     2581 gctgcacatc aaggtgctaa tagaacctcc tctctagaaa atcatgacca aatgtctgtg
     2641 aactgcagaa cgttgctcag cgagtcccct ggtaagctcc acgttgaagt ttcagcagat
     2701 cttacagcaa ttaatggtct ttcttacaat caaaatctcg ccaatggcag ttatgattta
     2761 gctaacagag acttttgcca aggaaatcag ctaaattact acaagcagga aatacccgtg
     2821 caaccaacta cctcatattc cattcagaat ttatacagtt acgagaacca gccccagccc
     2881 agcgatgaat gtactctcct gagtaataaa taccttgatg gaaatgctaa caaatctacc
     2941 tcagatggaa gtcctgtgat ggccgtaatg cctggtacga cagacactat tcaagtgctc
     3001 aagggcagga tggattctga gcagagccct tctattgggt actcctcaag gactcttggc
     3061 cccaatcctg gacttattct tcaggctttg actctgtcaa acgctagtga tggatttaac
     3121 ctggagcggc ttgaaatgct tggcgactcc tttttaaagc atgccatcac cacatatcta
     3181 ttttgcactt accctgatgc gcatgagggc cgcctttcat atatgagaag caaaaaggtc
     3241 agcaactgta atctgtatcg ccttggaaaa aagaagggac tacccagccg catggtggtg
     3301 tcaatatttg atccccctgt gaattggctt cctcctggtt atgtagtaaa tcaagacaaa
     3361 agcaacacag ataaatggga aaaagatgaa atgacaaaag actgcatgct ggcgaatggc
     3421 aaactggatg aggattacga ggaggaggat gaggaggagg agagcctgat gtggagggct
     3481 ccgaaggaag aggctgacta tgaagatgat ttcctggagt atgatcagga acatatcaga
     3541 tttatagata atatgttaat ggggtcagga gcttttgtaa agaaaatctc tctttctcct
     3601 ttttcaacca ctgattctgc atatgaatgg aaaatgccca aaaaatcctc cttaggtagt
     3661 atgccatttt catcagattt tgaggatttt gactacagct cttgggatgc aatgtgctat
     3721 ctggatccta gcaaagctgt tgaagaagat gactttgtgg tggggttctg gaatccatca
     3781 gaagaaaact gtggtgttga cacgggaaag cagtccattt cttacgactt gcacactgag
     3841 cagtgtattg ctgacaaaag catagcggac tgtgtggaag ccctgctggg ctgctattta
     3901 accagctgtg gggagagggc tgctcagctt ttcctctgtt cactggggct gaaggtgctc
     3961 ccggtaatta aaaggactga tcgggaaaag gccctgtgcc ctactcggga gaatttcaac
     4021 agccaacaaa agaacctttc agtgagctgt gctgctgctt ctgtggccag ttcacgctct
     4081 tctgtattga aagactcgga atatggttgt ttgaagattc caccaagatg tatgtttgat
     4141 catccagatg cagataaaac actgaatcac cttatatcgg ggtttgaaaa ttttgaaaag
     4201 aaaatcaact acagattcaa gaataaggct taccttctcc aggcttttac acatgcctcc
     4261 taccactaca atactatcac tgattgttac cagcgcttag aattcctggg agatgcgatt
     4321 ttggactacc tcataaccaa gcacctttat gaagacccgc ggcagcactc cccgggggtc
     4381 ctgacagacc tgcggtctgc cctggtcaac aacaccatct ttgcatcgct ggctgtaaag
     4441 tacgactacc acaagtactt caaagctgtc tctcctgagc tcttccatgt cattgatgac
     4501 tttgtgcagt ttcagcttga gaagaatgaa atgcaaggaa tggattctga gcttaggaga
     4561 tctgaggagg atgaagagaa agaagaggat attgaagttc caaaggccat gggggatatt
     4621 tttgagtcgc ttgctggtgc catttacatg gatagtggga tgtcactgga gacagtctgg
     4681 caggtgtact atcccatgat gcggccacta atagaaaagt tttctgcaaa tgtaccccgt
     4741 tcccctgtgc gagaattgct tgaaatggaa ccagaaactg ccaaatttag cccggctgag
     4801 agaacttacg acgggaaggt cagagtcact gtggaagtag taggaaaggg gaaatttaaa
     4861 ggtgttggtc gaagttacag gattgccaaa tctgcagcag caagaagagc cctccgaagc
     4921 ctcaaagcta atcaacctca ggttcccaat agctgagtcg agtctagagg gcccgtttaa
     4981 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     5041 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     5101 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     5161 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     5221 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     5281 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5341 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5401 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5461 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5521 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5581 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5641 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     5701 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5761 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     5821 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     5881 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5941 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     6001 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     6061 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     6121 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     6181 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     6241 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     6301 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     6361 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     6421 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     6481 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     6541 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     6601 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     6661 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     6721 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6781 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6841 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6901 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     6961 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     7021 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     7081 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7141 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7201 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     7261 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7321 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7381 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7441 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7501 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7561 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7621 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7681 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7741 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7801 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7861 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7921 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7981 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8041 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8101 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8161 caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8221 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8281 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8341 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8401 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8461 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8521 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8581 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8641 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8701 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8761 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8821 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8881 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8941 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9001 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9061 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9121 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9181 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9241 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9301 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9361 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
