LOCUS       sequence-78281-e       10762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10762)
  AUTHORS   Kwon SC, Yi H, Eichelbaum K, Fohr S, Fischer B, You KT, Castello A, 
            Krijgsveld J, Hentze MW, Kim VN
  TITLE     The RNA-binding protein repertoire of embryonic stem cells.
  JOURNAL   Nat Struct Mol Biol. 2013 Sep;20(9):1122-30. doi: 10.1038/nsmb.2638.
            Epub 2013 Aug 4.
  PUBMED    23912277
REFERENCE   2  (bases 1 to 10762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2638";
            journalName: "Nat Struct Mol Biol"; date: "2013-09"; volume: "20"; 
            issue: "9"; pages: "1122-30"
COMMENT     Sequence Label: pLVX-3XMyc-mTrim25
FEATURES             Location/Qualifiers
     source          1..10762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2337..3519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2568..3510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3467..3487
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3580..3669
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     promoter        5597..6096
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5632..5655)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6029..6048
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6117..6716
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6117..6136)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6613..6633
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6730..7318
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6783..6803)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7201..7212)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7525..8158
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8287..8303)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8287..8303)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8300..8322)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8311..8327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8335..8341,8342..8359,8360..8365))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8380..8401
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8518..8535)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8689..9277)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8769..8788)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9448..10239,10240..10308))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10071..10090
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10309..10413)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10461..10595
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10498..10517)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10552..10571
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatcgctagc gctaccggac
     3541 tcagatctcg agctcaagct tcgaattgga tctaacatgg aacaaaaact catctcagaa
     3601 gaggatctgg aacaaaaact catctcagaa gaggatctgg aacaaaaact catctcagaa
     3661 gaggatctgg gatccatggc ggagctgaat cctctggccg aggagctgtc ctgctccgtc
     3721 tgcctggagc tcttcaagga acctgtcacc accccgtgcg gccacaactt ctgcatgtca
     3781 tgcctggatg agacgtgggt cgtccagggc ccgccgtacc gatgcccgca atgtcgcaaa
     3841 gtgtaccagg tgcgcccgca gctgcaaaag aacacggtaa tgtgcgcggt ggtggagcag
     3901 ttcctgcagg ccgagcaggc gcggaccccg gtggacgact ggacgcctcc tgcccgcttc
     3961 tccgcctcca gcgcagccac ccaggtggcc tgcgaccact gcctgacgga gatcgcagtg
     4021 aagacgtgcc tcgtgtgcat ggcctctttc tgccaggagc acttgaggcc tcacttcgac
     4081 agtccggcct tccaggatca ccccctacag tcgcccatcc gtgaccttct gcgccgcaaa
     4141 tgtacccagc acaaccgcct tcgggaattc ttttgtcccg agcatggcga gtgcatctgc
     4201 catatctgct tggtagagca caagacctgc tcccctacga ccctaagtca agccagcgcc
     4261 gacctggagt acaaactgag gaataaactc actatcatgc acagccacat caatggggca
     4321 accaaagcat tggaggatgt gagatccaag caacagtgtg tgcaggattc tatgaagagg
     4381 aagatggaac agctgagaca ggagtatatg gaaatgaagg ctgtcatcga cgcagcagag
     4441 accagctcct tgcgcaggtt gaaggaggag gagaagagag tctacggaaa attcgacacc
     4501 atctaccagg ttctcgtcaa gaagaagagt gagatgcaga aactgaaggc agaggttgag
     4561 ctcattatgg acaaagggga tgagtttgag tttctggaga aagctgcaaa attgcaagga
     4621 gagtcaacaa aaccagtcta catccctaag atagatctgg atcatgattt gataatggga
     4681 atctaccaag gggcggcgga cctcaagagt gaactgaagc actccattaa gaagctacag
     4741 aagaagtctg aggagcacaa tggctcaggt aacaagggag atcagacaca gtccactttt
     4801 aaacccgtac aaccttcgaa gaagaccatt caagaaaaga agaccaagaa aacccctgtt
     4861 gctcccggcc ctccctccca cttctcaccc aacaagttgc ccacctttgg agctcctggc
     4921 caatcactgg attcaaaagc aacttcccct gatgccgcac ccaaagccag tgctgcacag
     4981 ccagactcta taggagtcaa ggctaaggtg ctagaaaact tcttaaccaa gtccagaacg
     5041 gagcttctgg agtatttcgt gaaagtcatc ttcgactaca ataccgccca caacaaagtg
     5101 tctctgtcaa acaagtacac caccgcctct gtttctgatg ggctccagca ctaccggtcc
     5161 catccccaga ggttcaccta ctgctctcag gtcctggggc tgcactgcta caagaatggc
     5221 attcactact gggaggtgga actacagaag aacaacttct gcggcgtagg catctgctac
     5281 ggcagcatgg aacggcaggg ccccgagagc cggctgggcc gcaaccccaa ctcatggtgt
     5341 gtggagtggt tcaataacaa gatctccgcc tggcacaaca acgtggagaa gactctgccc
     5401 tccaccaagg ccactcgagt cggcgtgctg ctcaactgtg accacggctt cgtcattttc
     5461 tttgctgtca ccgaaaaggt gcacctgatg tacaagttca aggtggactt caccgaagct
     5521 ctgtacccag ccttctgggt gttctcggcg ggtaccaccc tctctatctg ctccaaatag
     5581 tctagataat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     5641 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     5701 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     5761 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     5821 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     5881 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     5941 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     6001 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     6061 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga
     6121 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     6181 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     6241 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     6301 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     6361 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     6421 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     6481 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     6541 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     6601 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     6661 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaccgc
     6721 gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6781 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6841 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6901 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6961 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7021 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     7081 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     7141 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7201 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7261 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     7321 ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     7381 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     7441 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7501 actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata
     7561 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     7621 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     7681 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     7741 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     7801 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     7861 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     7921 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     7981 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8041 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8101 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8161 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     8221 agtgagaggc cttgacattg ctagcgtttt accgtcgacc tctagctaga gcttggcgta
     8281 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8341 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8401 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8461 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8521 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8581 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8641 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8701 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8761 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8821 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8881 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8941 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9001 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9061 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9121 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9181 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9241 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9301 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9361 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9421 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9481 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9541 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9601 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9661 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9721 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9781 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9841 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9901 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9961 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10021 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10081 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10141 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10201 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10261 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10321 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10381 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    10441 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca
    10501 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    10561 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
    10621 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
    10681 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    10741 tatgtactac aaacttagta gt
//
