LOCUS       sequence-78282-e       10304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10304)
  AUTHORS   Kwon SC, Yi H, Eichelbaum K, Fohr S, Fischer B, You KT, Castello A, 
            Krijgsveld J, Hentze MW, Kim VN
  TITLE     The RNA-binding protein repertoire of embryonic stem cells.
  JOURNAL   Nat Struct Mol Biol. 2013 Sep;20(9):1122-30. doi: 10.1038/nsmb.2638.
            Epub 2013 Aug 4.
  PUBMED    23912277
REFERENCE   2  (bases 1 to 10304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2638";
            journalName: "Nat Struct Mol Biol"; date: "2013-09"; volume: "20"; 
            issue: "9"; pages: "1122-30"
COMMENT     Sequence Label: pLVX-3XMyc-mTriml1
FEATURES             Location/Qualifiers
     source          1..10304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2337..3519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2568..3510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3467..3487
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3580..3669
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     promoter        5139..5638
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5174..5197)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5571..5590
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5659..6258
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5659..5678)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6155..6175
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6272..6860
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6325..6345)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6743..6754)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7067..7700
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7829..7845)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7829..7845)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7842..7864)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7853..7869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7877..7883,7884..7901,7902..7907))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7922..7943
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8060..8077)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8231..8819)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8311..8330)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8990..9781,9782..9850))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9613..9632
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9851..9955)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10003..10137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10040..10059)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10094..10113
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatcgctagc gctaccggac
     3541 tcagatctcg agctcaagct tcgaattgga tctaacatgg aacaaaaact catctcagaa
     3601 gaggatctgg aacaaaaact catctcagaa gaggatctgg aacaaaaact catctcagaa
     3661 gaggatctgg gatcctgtgc tggaattctg atgtccaacc atgagaaaat gtccacaaca
     3721 gatttgatgg agaacctcag ggaagagctg acctgtttca tctgcttgga ttatttcagc
     3781 agtccagtga ccaccgagtg tgggcacagc ttttgtctga tgtgcctcct caagagttgg
     3841 gaggagcaca acacaccttt atcttgtcct gaatgctgga ggactttggg agccccacat
     3901 tttcaagcca acgagcggct ggggagactg gctaatattg gcagacagct gagatcgcag
     3961 gtgctgcaga gcgaggatga gcagagcatc tgtgggagaa tgccagggcc tagttgggtg
     4021 ttctctgacg atgagcagag tgtcattaat gtctcacctc caagtcaagg gacgaacaag
     4081 gcgtgtttct caagtgaggc tgaggagcag cacaaagaaa aactccaaga tatcataaat
     4141 attttacgga aaaagaagaa ggaagttcag gccatcctga accatgagaa ggaacgagtg
     4201 atgttgtgta aggaggagac aaagacttgt aagcaggtcg ttgtgtctga atacatgaag
     4261 atgcaccagt tcctcaagga agaggaacag ctccagctcc agctcctgga gagggaggag
     4321 aaggcgaaca tgaagaagct gagggaaaat gagatccagc tgacccagca aatccgccgc
     4381 ctcggcaaaa tgattgggcg gattgagtct acctgtcaga acttgacgtt agaatctttt
     4441 gaggaagtca aaggagccat ggatcggtat gagtccctgt tgtttcagtc tccagagacc
     4501 accatcacgg aactgagcct gtgtcacatc actgggatga gggagatgct gcgaaaattc
     4561 agcacagata taactctaga tccagccaca gccaatgctt acctgctttt gtccgaagat
     4621 ctgaaaagtg tgcggtatgg agggaccagg cagcaattac ctgacaaccc agagcgattt
     4681 gatcagtctg caactgtgtt gggagctcag atcttcacct gcgggagaca ctactgggaa
     4741 gtggaagtgg gaaagaagac ggagtgggaa gtggggatct gcaaggactc tgtgaacaga
     4801 aaggggaatc tccccaaacc gcccggggac ctcttctcac tcataggctt aaaaattggc
     4861 gacgattaca gtctctgggt ttcatctccc ctgaaagggc aacatgtccg agaacccgtg
     4921 cataaagttg gtgtgttctt ggactatgac tctggacaca tagccttcta caatgcgaca
     4981 gatgagtccc tcatctacag cttccctccg acccctttcc atgaagctct cagacctatc
     5041 ttttcccctt gtcttccgaa tgaggggacg aatacagacc ctcttatcat ctgccacatc
     5101 tgagcggccg ctcccgggct catctagata attctaccgg gtaggggagg cgcttttccc
     5161 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     5221 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     5281 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     5341 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     5401 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     5461 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     5521 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
     5581 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
     5641 ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     5701 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     5761 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     5821 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     5881 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     5941 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6001 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6061 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6121 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6181 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6241 cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca aaatttgtga
     6301 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6361 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6421 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6481 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6541 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6601 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6661 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6721 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6781 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6841 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     6901 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     6961 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     7021 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     7081 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     7141 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     7201 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     7261 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     7321 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     7381 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     7441 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     7501 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7561 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7621 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7681 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7741 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt ttaccgtcga
     7801 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7861 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7921 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7981 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8041 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8101 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8161 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8221 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8281 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8341 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8401 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8461 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8521 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8581 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8641 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8701 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8761 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8821 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8881 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8941 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9001 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9061 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9121 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9181 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9241 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9301 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9361 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9421 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9481 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9541 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9601 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9661 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9721 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9781 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9841 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9901 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9961 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
    10021 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    10081 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    10141 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    10201 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    10261 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
