LOCUS       sequence-78297-e        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized SpCas9 and chimeric guide RNA expression 
            plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, 
            Jiang W, Marraffini LA, Zhang F
  TITLE     Multiplex Genome Engineering Using CRISPR/Cas Systems.
  JOURNAL   Science. 2013 Jan 3.
  PUBMED    23287718
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Jan 3."
COMMENT     Sequence Label: pX330-U6-Chimeric_BB-CBh-hSpCas9
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      59..68
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             68..133
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             140..160
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 taatgtttaa ttacctggag cacctgcctg aaatcacttt ttttcaggtt ggaccggtgc
       61 caccatggac tataaggacc acgacggaga ctacaaggat catgatattg attacaaaga
      121 cgatgacgat aagatggccc caaagaagaa gcggaaggtc ggtatccacg gagtcccagc
      181 agccgacaag aagtacagca tcggcctgga catcggcacc aactctgtgg gctgggccgt
      241 gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg
      301 gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga
      361 ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg
      421 ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag
      481 actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg
      541 caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa
      601 gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca
      661 catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga
      721 cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc
      781 catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag
      841 acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggaaa
      901 cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga
      961 ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc
     1021 ccagatcggc gaccagtacg ccgacctgtt tc
//
