LOCUS       sequence-78304-e        1109 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized SpCas9 and chimeric guide RNA expression 
            plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, 
            Jiang W, Marraffini LA, Zhang F
  TITLE     Multiplex Genome Engineering Using CRISPR/Cas Systems.
  JOURNAL   Science. 2013 Jan 3.
  PUBMED    23287718
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Jan 3."
COMMENT     Sequence Label: pX330-U6-Chimeric_BB-CBh-hSpCas9
FEATURES             Location/Qualifiers
     source          1..1109
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gccttatcca gttcgctcag gccgcctctc tcggccttgg tcagattgtc gaactttctc
       61 tgggtaatca gcttggcgtt cagcagctgc cgccagtagt tcttcatctt cttcacgacc
      121 tcttcggagg gcacgttgtc gctcttgccc cggttcttgt cgcttctggt cagcaccttg
      181 ttgtcgatgg agtcgtcctt cagaaagctc tgaggcacga tatggtccac atcgtagtcg
      241 gacagccggt tgatgtccag ttcctggtcc acgtacatat cccgcccatt ctgcaggtag
      301 tacaggtaca gcttctcgtt ctgcagctgg gtgttttcca cggggtgttc tttcaggatc
      361 tggctgccca gctctttgat gccctcttcg atccgcttca ttctctcgcg gctgttcttc
      421 tgtcccttct gggtggtctg gttctctctg gccatttcga tcacgatgtt ctcgggcttg
      481 tgccggccca tcactttcac gagctcgtcc accaccttca ctgtctgcag gatgcccttc
      541 ttaatggcgg ggctgccggc cagattggca atgtgctcgt gcaggctatc gccctggccg
      601 gacacctggg ctttctggat gtcctcttta aaggtcaggc tgtcgtcgtg gatcagctgc
      661 atgaagtttc tgttggcgaa gccgtcggac ttcaggaaat ccaggattgt cttgccggac
      721 tgcttgtccc ggatgccgtt gatcagcttc cggctcagcc tgccccagcc ggtgtatctc
      781 cgccgcttca gctgcttcat cactttgtcg tcgaacaggt gggcataggt tttcagccgt
      841 tcctcgatca tctctctgtc ctcaaacagt gtcagggtca gcacgatatc ttccagaatg
      901 tcctcgtttt cctcattgtc caggaagtcc ttgtccttga taattttcag cagatcgtgg
      961 tatgtgccca gggaggcgtt gaaccgatct tccacgccgg agatttccac ggagtcgaag
     1021 cactcgattt tcttgaagta gtcctctttc agctgcttca cggtcacttt ccggttggtc
     1081 ttgaacagca ggtccacgat ggccttttt
//
