LOCUS       sequence-78307-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Yamada KM
  TITLE     Yamada Lab Plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pmyr GFP
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(37..57)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     regulatory      54..63
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(109..128)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(141..159)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(142..159)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(369..393)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(390..593)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(419..439)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        594..897
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cccttgctca ccatgcgctg gctggggtcc cttaggcttg ctcttgctgc tccccatggt
       61 ggcggctgaa tcgatagaac cgaggtgcag ttggacctgg gagtggacac ctgtggagag
      121 aaaggcaaag tggatgttat tctatagtgt cacctaaatc gtatgtgtat gatacataag
      181 gttatgtatt aattgtagcc gcgttctaac gaagccaagg gggtgggcct atagactcta
      241 taggcggtac ttacgtcact cttggcacgg ggaatccgcg ttccaatgca ccgttcccgg
      301 ccgcggaggc tggatcggtc ccggtgtctt ctatggaggt caaaacagcg tggatggcgt
      361 ctccaggcga tctgacggtt cactaaacga gctctgctta tatagacctc ccaccgtaca
      421 cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt
      481 tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc
      541 cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta
      601 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac
      661 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc
      721 atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga
      781 cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat
      841 gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg
      901 aactccatat atgggctatg aactaatgac cccgtaa
//
