LOCUS       sequence-78316-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Right TALEN for PKP2 with NN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga 
            PP, So PL, Conklin BR
  TITLE     Isolation of single-base genome-edited human iPS cells without 
            antibiotic selection.
  JOURNAL   Nat Methods. 2014 Mar;11(3):291-3. doi: 10.1038/nmeth.2840. Epub 
            2014 Feb 9.
  PUBMED    24509632
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2840"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "291-3"
COMMENT     Sequence Label: MR015-PKP2 Right TALEN_NN
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      90..99
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..164
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             171..191
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctggctagcg ccaccatgga ctacaaagac catgacggtg
      121 attataaaga tcatgacatc gattacaagg atgacgatga caagatggcc cccaagaaga
      181 agaggaaggt gggcattcac cgcggggtac ctatggtgga cttgaggaca ctcggttatt
      241 cgcaacagca acaggagaaa atcaagccta aggtcaggag caccgtcgcg caacaccacg
      301 aggcgcttgt ggggcatggc ttcactcatg cgcatattgt cgcgctttca cagcaccctg
      361 cggcgcttgg gacggtggct gtcaaatacc aagatatgat tgcggccctg cccgaagcca
      421 cgcacgaggc aattgtaggg gtcggtaaac agtggtcggg agcgcgagca cttgaggcgc
      481 tgctgactgt ggcgggtgag cttagggggc ctccgctcca gctcgacacc gggcagctgc
      541 tgaagatcgc gaagagaggg ggagtaacag cggtagaggc agtgcacgcc tggcgcaatg
      601 cgctcaccgg tgcccccctg aacctgaccc cggaccaagt ggtggctatc gccagcaaca
      661 atggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc
      721 atggcctgac cccggaccaa gtggtggcta tcgccagcaa caatggcggc aagcaagcgc
      781 tcgaaacggt gcagcggctg ttgccggtgc tgtgcca
//
