LOCUS       sequence-78328-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of VSV-tagged Cortactin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Yamada KM
  TITLE     Yamada Lab Plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pRK VSV Cortactin
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        235..253
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     235..252
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     266..285
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      331..340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             343..375
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             376..408
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
ORIGIN
        1 gagctcgttt agtgaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
       61 tagaagacac cgggaccgat ccagcctccg cggccgggaa cggtgcattg gaacgcggat
      121 tccccgtgcc aagagtgacg taagtaccgc ctatagagtc tataggccca cccccttggc
      181 ttcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag
      241 gtgacactat agaataacat ccactttgcc tttctctcca caggtgtcca ctcccaggtc
      301 caactgcacc tcggttctat cgattcagcc gccaccatgg gatacaccga catcgagatg
      361 aaccggctgg gcaagtacac cgacatcgag atgaaccggc tgggcaaggg atccatgtgg
      421 aaagcttcag caggccacgc tgtgtccatc gcccaggatg acgcgggggc cgatgactgg
      481 gagaccgacc ctgattttgt gaatgatgtg agtgagaagg agcaaagatg gggtgccaag
      541 acggtgcagg gctccgggca ccaggagcat atcaacatac acaagctgag ggagaatgtc
      601 tttcaagagc atcagaccct taaggagaag gaacttgaaa caggaccaaa agcttcccat
      661 ggctatggag ggaaatttgg tgtggaacaa gaccgaatgg ataagtcagc tgtcggccac
      721 gaatatcagt cgaaactttc caagcactgc tcgcaggtgg actcggtccg tggcttcgga
      781 ggcaagtttg gtgtccagat ggacagagtt gatcagtctg ctgtaggctt tgaataccag
      841 gggaagactg agaagcatgc ctcccagaaa gactactcca gtggttttgg cggcaagtat
      901 ggcgtgc
//
