LOCUS       sequence-78336-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Dunlop MJ, Dossani ZY, Szmidt HL, Chu HC, Lee TS, Keasling JD, Hadi 
            MZ, Mukhopadhyay A
  TITLE     Engineering microbial biofuel tolerance and export using efflux 
            pumps.
  JOURNAL   Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
  PUBMED    21556065
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2011-05-10"; volume: "7:487. doi"; pages: " 
            10.1038/msb.2011.21"
COMMENT     Sequence Label: pBbA5k-EPL45
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      210..219
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtggtgatgg tgatgatggg ccagttgctg cagttcctcc agctcgctgg agcgactgtg
       61 ggcgcgtgcc atcaggctgt agatcgacgg cagcacgaac agggtgaaca gggtgcccac
      121 cagcatgccg atgacgatca ccagccccag gccgaaacgg ctgctggcgc cggcgccgga
      181 agaaaacagc agcggcacca gccccaccac catggcggcg gtggtcatga ggatcggccg
      241 caggcggatc cgtgccgcgc ggataatcgc ctgggccgca gtgcagccca gggcggcgcg
      301 cagttcattg gcgaactcca ccatcaggat gccgtgcttg ctgatgaggc cgatcagggt
      361 caccaggccg atctgcgtgt agatattgat ggtcgccagc cccagcgcca ggggaatcag
      421 cgcgccacag atcgacagcg gcacagtgat caggatgatc agcgggtcgc tcaggctttc
      481 gtactgcgcg gccagcacca tgtagatcac gatgagggcg aacaggaaag tcagcagcag
      541 ggcattgcct tcgtggatga actgccgcga atcggcctgc cagtcatggc tgaaactggc
      601 gggcagggcc tggctttgct gctgcaggaa ggccaccgcc tggcccaggc tgacccccgg
      661 ggcggggatg ccctggaagg tcgcggcatt ctgctggttg aactgcttga gcttgttggg
      721 ctggatgctg tcatgcaggc tgaccacggt ggacaacggc acctgggtgc cgctgtcggt
      781 ctccacgtag aagccgttga gggcctgggg tgtc
//
