LOCUS       sequence-78341-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Dunlop MJ, Dossani ZY, Szmidt HL, Chu HC, Lee TS, Keasling JD, Hadi 
            MZ, Mukhopadhyay A
  TITLE     Engineering microbial biofuel tolerance and export using efflux 
            pumps.
  JOURNAL   Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
  PUBMED    21556065
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2011-05-10"; volume: "7:487. doi"; pages: " 
            10.1038/msb.2011.21"
COMMENT     Sequence Label: pBbA5k-EPL54
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtggtgatgg tgatgatgct ccttgttacg ggtttccggt tgactcaggc ttgcctgagg
       61 cgcctgtgct gcttccgact ccacgggcca gccaccaccc agcgccttgt agagctggac
      121 aagttggcca aactccgaac caatgagggt ggactggtcg agttgcgcgg cgaacagcgc
      181 gcgctcggaa tccagcactt caagatagct cgtataaccg ccttcgtagc gatctcgtgc
      241 cagcgaagcg taacggccca gcgcatcgac ctggtcggtc ttggcattga gctgatcgcg
      301 cgtacgctgc acgctgacca gcgcatccgc cacttcccgg aacgctgtct ggatggattg
      361 ctggtaggcc tgcagcgact gcgtctgaat cgcctccgcc tgcttgacgt cgccggcgat
      421 cgcgccaccg gtgaagatcg gcatactgat gagccctgcg aacgaccagg cccgtgccgg
      481 accggtccac agccccgaca gtgccgtgct ggcagcgccg aacgcgccgg tcagcgaaat
      541 cgtcgggaag tattcggcac gcgcggcgcc gatgcgggca ttggccgcca cgagattctg
      601 ctcggccagg agcacgtccg gccgctgctc cagtaacgtc gaaggcaggc cgaccggcac
      661 gcccggcaag gtcaactcgg agatcgactt gccgcgcgga atttcacgcg gattctcgcc
      721 cagcagaccg gccagcagat cctcctgcag cgcgacttgc tggcggaact gctcgaccga
      781 ggtcagtgcc gatgcgtatt ccgaccgcgc ttgcgcgaac tcgacctcgg aga
//
