LOCUS       sequence-78345-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Dunlop MJ, Dossani ZY, Szmidt HL, Chu HC, Lee TS, Keasling JD, Hadi 
            MZ, Mukhopadhyay A
  TITLE     Engineering microbial biofuel tolerance and export using efflux 
            pumps.
  JOURNAL   Mol Syst Biol. 2011 May 10;7:487. doi: 10.1038/msb.2011.21.
  PUBMED    21556065
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2011-05-10"; volume: "7:487. doi"; pages: " 
            10.1038/msb.2011.21"
COMMENT     Sequence Label: pBbA5k-EPL63
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtggtgatgg tgatgatgct ccctgagggt caaacctgtt tcggcattcc agccgccacc
       61 caatgcggca atcaactgaa cgctggcaat caagcgactt tgcagaacgt tcagcacact
      121 gcgttcattg ctcaaagcag tggcctgtac gttgacgacg tcaagataac cgatcagccc
      181 ggccttgtac tggttgttgg tcaggcgcaa cgagtcgcga gccgatacca gcgcttcctg
      241 gcgcacggcg gcctcctgct cgtagacctt gagttgaacg aggtaatttt cgacttcctg
      301 aaaaccattc agcacggtct gccggtactg ggcgacagtc tggtcgtaca cggcctcggt
      361 acggtcaact tccgccgtcc gcctgccacc gtcgaacaac gtcaacgcca gttgcggccc
      421 taccgaccag aagcggttgg gcaggctgat ccagttggcg aacgtactgc tgctgtaacc
      481 gccgctcatg ctcagcgtga agtctgggta gtaagcggcc ttggcgacgc cgatgttggc
      541 attggcggcc atgaccgagc gttcggccgc ggcaatgtcc ggtcggcgct caagcaactg
      601 tgacggcagc cctggcggga tctgcggcaa ttgcggaatc gaagtgctgg ccggcagatt
      661 gaagtcagcg ggcgccatac ccaccagcac ggcaatggcg ttctcgaatt gtgcacgctg
      721 ccaggccaga tcgatcaggt cggcctgagt acttttcaac tgcgtctgcg cctgggcgac
      781 agcgtcactt cctgaaatac cggcgcggta ttgattctgg gtgagggtca gagagcgctg
      841 ataggtgtcc acagtg
//
