LOCUS       sequence-78348-e         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for the Golden Gate TALEN kit, for in vitro 
            synthesis of TALEN mRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Carlson DF, Tan W, Lillico SG, Stverakova D, Proudfoot C, Christian 
            M, Voytas DF, Long CR, Whitelaw CB, Fahrenkrug SC
  TITLE     Efficient TALEN-mediated gene knockout in livestock.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Oct 1.
  PUBMED    23027955
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Oct 1."
COMMENT     Sequence Label: RCIscript-GoldyTALEN
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(51..638)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 ttgaggctgg tttagtggta accagatcct agtcagtcac tagcctatta aaagtttatc
       61 tcaccgttat taaatttcct tctcacttcc tcaagtgtca atgtaccagc tttaatcatt
      121 tctccaccaa tcaaaagctc ttctacacta agaacagctc cattacaatt agtgatatga
      181 ttcaatcttg taagctgagc tttgtagttt cctttgaaat gaccactcac aaacaagaac
      241 ttaaattctg ttactgaaga tggatagact ttccaccatt cattagggtt gatatgcttg
      301 tttcttgttt gattctcttc gacatatctt tgcatttcat ctgcttgacc aattggaaga
      361 ttataacctc ctgaatatgc cttagtatca acgatcacac cgtaatcaat aggagatccg
      421 acagtataaa ttgctccgtc tggtttcctt gatccaccca aatgtttacc acgataacca
      481 taaaccttca taaagaactc catcaccttc atttcaagga ttctatcctg agttgaattt
      541 cttgcgattt caatcaattc aatatattca tgtggcacat atttcaattt atgtctaagt
      601 tcagatttct tctcttccaa ttcagatttc actagctggg atctagaggc aacgcgatgg
      661 gacgtgcgtt cgccaatacg gcgattgact cttctgatca attccggcgc gtgcggca
//
