LOCUS       sequence-78349-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destination vector for the Golden Gate TALEN kit, for in vitro 
            synthesis of TALEN mRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Carlson DF, Tan W, Lillico SG, Stverakova D, Proudfoot C, Christian 
            M, Voytas DF, Long CR, Whitelaw CB, Fahrenkrug SC
  TITLE     Efficient TALEN-mediated gene knockout in livestock.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Oct 1.
  PUBMED    23027955
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Oct 1."
COMMENT     Sequence Label: RCIscript-GoldyTALEN
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             202..789
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gctactcaca ttaattgcgt tgcgctcact gcccgctttc caccggtcgt ctccaacgac
       61 cacctcgtcg ccttggcctg cctcggcgga cgtcctgcca tggatgcagt gaaaaaggga
      121 ttgccgcacg cgccggaatt gatcagaaga gtcaatcgcc gtattggcga acgcacgtcc
      181 catcgcgttg cctctagatc ccagctagtg aaatctgaat tggaagagaa gaaatctgaa
      241 cttagacata aattgaaata tgtgccacat gaatatattg aattgattga aatcgcaaga
      301 aattcaactc aggatagaat ccttgaaatg aaggtgatgg agttctttat gaaggtttat
      361 ggttatcgtg gtaaacattt gggtggatca aggaaaccag acggagcaat ttatactgtc
      421 ggatctccta ttgattacgg tgtgatcgtt gatactaagg catattcagg aggttataat
      481 cttccaattg gtcaagcaga tgaaatgcaa agatatgtcg aagagaatca aacaagaaac
      541 aagcatatca accctaatga atggtggaaa gtctatccat cttcagtaac agaatttaag
      601 ttcttgtttg tgagtggtca tttcaaagga aactacaaag ctcagcttac aagattgaat
      661 catatcacta attgtaatgg agctgttctt agtgtagaag agcttttgat tggtggagaa
      721 atgattaaag ctggtacatt gacacttgag gaagtgagaa ggaaatttaa taacggtgag
      781 ataaactttt aata
//
