LOCUS       sequence-78366-e       10467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses codon optimized human L1 retrotransposon driven by CMV 
            promoter and tagged with the self splicing intron neo cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10467)
  AUTHORS   Kroutter EN, Belancio VP, Wagstaff BJ, Roy-Engel AM
  TITLE     The RNA polymerase dictates ORF1 requirement and timing of LINE and 
            SINE retrotransposition.
  JOURNAL   PLoS Genet. 2009 Apr;5(4):e1000458. doi: 
            10.1371/journal.pgen.1000458. Epub 2009 Apr 24.
  PUBMED    19390602
REFERENCE   2  (bases 1 to 10467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2009-04"; volume: "5(4):e1000458. doi"; pages: " 
            10.1371/journal.pgen.1000458. Epub 2009 Apr 24"
COMMENT     Sequence Label: L1-neo-TET
FEATURES             Location/Qualifiers
     source          1..10467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        935..1314
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1315..1518
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1469..1489
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1515..1539
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(6619..6638)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7642..7661
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      7733..7868
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7738..8035)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     8033..8053
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    8079..8200
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8090..8109)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8144..8163
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8279..8299)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8279..8297)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8318..8334)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8318..8334)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8331..8353)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8342..8358
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8366..8372,8373..8390,8391..8396))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8411..8432
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8549..8566)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8720..9308)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8800..8819)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9479..10270,10271..10339))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10102..10121
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10340..10444)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gagagcacgc gttgccaagc tagtcgacca attctcatgt ttgacagctt
      721 atcatcgcag atccgggcaa cgttgttgca ttgctgcagg cgcagaactg gtaggtatgg
      781 aagatctata cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca
      841 taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt
      901 tatattggct catgtccaat atgaccgcca tgttgacatt gattattgac tagttattaa
      961 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     1021 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     1081 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     1141 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc
     1201 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     1261 cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     1321 cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     1381 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     1441 aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag
     1501 gtctatataa gcagagctcg tttagtgaac cgtcagatct ctagaagctg ggtaccagct
     1561 gctagctaaa accacaaaga tgggcaagaa gcagaaccgc aagaccggca acagcaagac
     1621 ccagagcgcc agcccccccc ccaaggagcg cagcagcagc cccgccaccg agcagagctg
     1681 gatggagaac gacttcgacg agctgcgcga ggagggcttc cgccgcagca actacagcga
     1741 gctgcgcgag gacatccaga ccaagggcaa ggaggtggag aacttcgaga agaacctgga
     1801 ggagtgcatc acccgcatca ccaacaccga gaagtgcctg aaggagctga tggagctgaa
     1861 gaccaaggcc cgcgagctgc gcgaggagtg ccgcagcctg cgcagccgct gcgaccagct
     1921 ggaggagcgc gtgagcgcaa tggaggacga gatgaacgag atgaagcgcg agggcaagtt
     1981 ccgcgagaag cgcatcaagc gcaacgagca gagcctccag gagatatggg actacgtgaa
     2041 gcgccccaac ctgcgcctga tcggcgtgcc cgagagcgac gtggagaacg gcaccaagct
     2101 ggagaacacc ctacaggaca tcatccagga gaacttcccc aacctggccc gccaggccaa
     2161 cgtgcagatc caggagatcc agcgcacccc ccagcgctac agcagccgcc gcgccacccc
     2221 ccgccacatc atcgtgcgct tcaccaaggt ggagatgaag gagaagatgc tgcgcgccgc
     2281 ccgcgagaag ggccgggtta ccctgaaggg caagcccatc cgcctgaccg ccgacctgag
     2341 cgccgagacc ctacaggccc gccgcgagtg ggggccaatc ttcaacatcc tgaaggagaa
     2401 gaacttccag ccccgcatca gctaccccgc caagctgagc ttcatcagcg agggcgagat
     2461 caaatacttc atcgacaagc agatgctgcg cgacttcgtg accacccgcc ccgccctgaa
     2521 ggagctgctg aaggaggccc tgaacatgga gcgcaacaac cggtatcagc ccctgcaaaa
     2581 ccacgccaag atgtaaagac catcaagact aggaagaaac tggatccact aatgagcaaa
     2641 atcaccagct aacgtctcaa tgaccggcag caccagccac atcaccatcc tgaccctgaa
     2701 catcaacggc ctgaacagcg ccatcaagcg gcaccggctg gccagctgga tcaagagcca
     2761 ggaccccagc gtgtgctgca tccaggagac ccacctgacc tgccgggaca cccaccggct
     2821 gaagattaag ggctggcgga agatctacca ggccaacggc aagcagaaga aggccggcgt
     2881 ggccatcctt gtcagcgaca agaccgactt caagcccacc aagatcaagc gggacaagga
     2941 gggccactac atcatggtga agggcagcat ccagcaggag gagctgacca tcctgaacat
     3001 ctacgccccc aacactggtg ccccccggtt catcaaacaa gttctgagcg accttcagcg
     3061 ggacctggac agccacaccc tgatcatggg cgacttcaac acccccctga gcaccctgga
     3121 ccggagcacc cggcagaagg tgaacaagga cacccaggag ctgaacagcg ccctgcacca
     3181 ggccgacctg atcgacatct accggaccct gcaccccaag agcaccgagt acaccttctt
     3241 cagcgccccc caccacacct acagcaagat cgaccacatc gtgggcagca aggccctgct
     3301 gagcaagtgc aagcggactg agatcatcac caactacctg agcgaccaca gcgccatcaa
     3361 gctggagctg cggatcaaga acctgaccca gagccggagc accacctgga agctgaacaa
     3421 cctgctgctg aacgactact gggttcacaa cgagatgaag gccgagatca agatgttctt
     3481 cgagaccaac gagaacaagg acaccaccta ccagaacctg tgggacgcct tcaaggccgt
     3541 gtgccggggc aagttcatcg ccctgaacgc ctacaagcgg aagcaggagc ggagcaagat
     3601 cgacaccctg accagccagc tgaaggagct ggagaagcag gagcagaccc acagcaaggc
     3661 cagtcggcgg caggagatca ccaagatccg ggccgagctg aaggagatcg agacccagaa
     3721 gacccttcag aagatcaacg agagccggag ctggttcttc gagcggatca acaagatcga
     3781 ccggcccctg gcccggctga tcaagaagaa gcgggagaag aaccagatcg acaccatcaa
     3841 gaacgacaag ggcgacatca ccaccgaccc caccgagatc cagaccacca tccgggagta
     3901 ctacaagcac ctgtacgcca acaagctgga gaatctagag gagatggaca ccttcctgga
     3961 cacctacacc ctgccccggc tgaaccagga ggaggtggag agcctgaacc ggcccatcac
     4021 cggcagcgag atcgtggcca tcatcaacag cctgcccacc aagaagagcc ccggccccga
     4081 cggcttcacc gccgagttct accagcggta caaggaggag ctggtgccct tcctgctgaa
     4141 gctgttccag agcatcgaga aggagggcat cctgcccaac agcttctacg aggccagcat
     4201 catcctgatc cccaagcccg gccgggacac caccaagaag gagaacttcc ggcccatcag
     4261 cctgatgaac atcgacgcca agatcctgaa caagatcctg gccaaccgga ttcagcagca
     4321 catcaagaag ctgatccacc acgaccaagt tggcttcatc cccggcatgc aaggatggtt
     4381 caacatccgg aagagcatca acgtgatcca gcacatcaac cgggccaagg acaagaacca
     4441 catgatcatc agcatcgacg ccgagaaggc tttcgacaag atccagcagc ccttcatgct
     4501 gaagaccctg aacaagctgg gcatcgacgg cacctacttc aagatcatcc gggccatcta
     4561 cgacaagccc accgccaaca tcatcctgaa cggccagaag ctggaggctt tccccctgaa
     4621 gaccggcacc cggcagggct gccccctgag ccccctgctg ttcaacatcg tgctggaggt
     4681 gctggcccgg gccatccggc aggagaagga gatcaagggc atccagctgg gcaaggagga
     4741 ggtgaagctg agcctgttcg ccgacgacat gatcgtgtac ctggagaacc ctattgttag
     4801 cgcccagaac ctgctgaagc tgatcagcaa cttcagtaaa gttagcggct acaagatcaa
     4861 cgtgcagaag agccaggctt tcctgtacac caataaccgg cagaccgaga gccagatcat
     4921 gggcgagctg cccttcacca tcgccagcaa gcggatcaag tacctgggca tccagctgac
     4981 tcgggacgtc aaggacctgt tcaaggagaa ctacaagccc ctgctgaagg agatcaagga
     5041 ggagaccaac aagtggaaga acatcccctg tagctgggtg ggccggatca acatcgtgaa
     5101 gatggccatc ctgccgaaag ttatctatcg gttcaacgcc atccccatca agctgcccat
     5161 gaccttcttc accgagctgg agaagaccac cctgaagttc atctggaacc agaagcgggc
     5221 ccggatcgcc aagagcatcc tgagccagaa gaacaaggcc ggcggcatca ccctgcccga
     5281 cttcaagctg tactacaagg ccaccgtgac caagaccgcc tggtactggt accagaaccg
     5341 ggacatcgac cagtggaacc ggaccgagcc cagcgagatc atgccccaca tctacaacta
     5401 cctgatcttc gacaagcccg agaagaacaa gcagtggggc aaggacagcc tgttcaacaa
     5461 gtggtgctgg gagaactggc tggccatctg ccggaagctg aagctggacc ccttcctgac
     5521 cccctacacc aagatcaaca gccggtggat caaggacctg aacgtgaagc ccaagaccat
     5581 caagaccctg gaggagaacc tgggcatcac catccaggac atcggcgtgg gcaaggactt
     5641 catgagcaag acccccaagg ccatggccac caaggacaag atcgacaagt gggacctgat
     5701 caagctgaag agcttctgca ccgccaagga gaccaccatc cgggtgaacc ggcagcccac
     5761 cacctgggag aagattttcg ccacctacag cagcgacaag ggcctgatca gccggatcta
     5821 caacgagctg aagcagattt acaagaagaa gaccaacaac cccatcaaga agtgggccaa
     5881 ggacatgaac cggcacttca gcaaggagga catctacgcc gccaagaagc acatgaagaa
     5941 gtgcagcagc agcctggcca tccgggagat gcagatcaag accaccatgc ggtatcacct
     6001 gacccccgtg cggatggcca tcatcaagaa gagcggcaac aaccgatgct ggcggggctg
     6061 cggcgagatc ggcaccctgc tgcactgctg gtgggactgc aagctggtgc agcccctgtg
     6121 gaagagcgtg tggcggttcc tgcgggacct ggagctggag atccccttcg accccgccat
     6181 ccccctgctg ggcatctacc ccaacgagta caagagctgc tgctacaagg acacctgcac
     6241 ccggatgttc atcgccgccc tgttcaccat cgccaagacc tggaaccagc ccaagtgccc
     6301 caccatgatc gactggatca agaagatgtg gcacatctac actatggagt actacgccgc
     6361 catcaagaac gacgagttca tcagcttcgt gggcacctgg atgaagctgg agaccatcat
     6421 cctgagcaag ctgagccagg agcagaagac caagcaccgg atcttcagcc tgatcggcgg
     6481 caactaagaa ttccgtctca aatcccctca gaagaactcg tcaagaaggc gatagaaggc
     6541 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
     6601 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
     6661 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattctcca ccatgatatt
     6721 cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt
     6781 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
     6841 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
     6901 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
     6961 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
     7021 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
     7081 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgtaattagc aatataatga
     7141 attgcaggga aaagttatca ggcatgcacc tggtagctag tctttaaacc aatagattgc
     7201 atcggtttaa aaggcaagac cgtcaaattg cgggaaaggg gtcaacagcc gttcagtacc
     7261 aagtctcagg ggaaactttg agatggcctt gcaaagggta tggtaataag ctgacggaca
     7321 tggtcctaac cacgcagcca agtcctaagt caacagatct tctgttgata tggatgcagt
     7381 tcacagacta aatgtcggtc ggggaagatg tattcttctc ataagatata gtcggacctc
     7441 tccttaatgg gagctagcgg atgaagtgat gcaacactgg agccgctggg acctaatttg
     7501 tatgcgaaag tatattgatt aggtttggag tactcgagtt cattcagggc accggacagg
     7561 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     7621 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     7681 ggagaacctg cgtgcaatcc atcttgttca atggccgatc ccatattggc tcgacctcca
     7741 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
     7801 taaataaaaa aaattagtca gccatggggc ggaaaatggg cggaactggg cggagttagg
     7861 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     7921 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     7981 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     8041 tgacacacat tccacagccg tctcgaattc agacatgata agatacattg atgagtttgg
     8101 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     8161 tgctttattt gtaaccatta taagctgcaa taaacaagtt ggggtgggcg ggccggccat
     8221 tgacatccaa acgagcgcta ttgcggccgc caccgcggtg gagctccagc ttttgttccc
     8281 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     8341 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8401 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     8461 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8521 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8581 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8641 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8701 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8761 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8821 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8881 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8941 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     9001 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9061 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9121 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     9181 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9241 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9301 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9361 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9421 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9481 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9541 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9601 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9661 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9721 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9781 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9841 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9901 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9961 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    10021 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    10081 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    10141 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    10201 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10261 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10321 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10381 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10441 cgcgcacatt tccccgaaaa gtgccac
//
