LOCUS       sequence-78383-e        3850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression of mouse Sox1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Nakagawa M, Koyanagi M, Tanabe K, Takahashi K, Ichisaka T, Aoi T, 
            Okita K, Mochiduki Y, Takizawa N, Yamanaka S
  TITLE     Generation of induced pluripotent stem cells without Myc from mouse 
            and human fibroblasts.
  JOURNAL   Nat Biotechnol. 2008 Jan;26(1):101-6. Epub 2007 Nov 30.
  PUBMED    18059259
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol."; date: "2008-01"; volume: "26(1)"; pages: "101-6. Epub 
            2007 Nov 30"
COMMENT     Sequence Label: pMXs-ms-Sox1
FEATURES             Location/Qualifiers
     source          1..3850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1100..1516
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1446..1468
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1484..1500
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1526..1900
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1838..1854
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1951..1975
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3188..3212)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaag cggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggatctcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct tagaggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat atgggcccgg gccagactgt taccactccc ttaagtttga ccttaggtca
     1141 ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg
     1201 ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac
     1261 ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg
     1321 acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc
     1381 ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc
     1441 tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac
     1501 tccttctcta ggcgccccca tatggccata tgagatctta tatggggcac ccccgcccct
     1561 tgtaaacttc cctgaccctg acatgacaag agttactaac agcccctctc tccaagctca
     1621 cttacaggct ctctacttag tccagcacga agtctggaga cctctggcgg cagcctacca
     1681 agaacaactg gaccgaccgg tggtacctca cccttaccga gtcggcgaca cagtgtgggt
     1741 ccgccgacac cagactaaga acctagaacc tcgctggaaa ggaccttaca cagtcctgct
     1801 gaccaccccc accgccctca aagtagacgg catcgcagct tggatacacg ccgcccacgt
     1861 gaaggctgcc gaccccgggg gtggaccatc ctctagactg ccggatctag ctagttaatt
     1921 aaggatccca gtgtggtggt acgggaaatc acaagtttgt acaaaaaagc aggctccgcg
     1981 gccgccccct tcaccatgta cagcatgatg atggagaccg acctgcactc gcccggcggc
     2041 gcccaggcgc ccacgaacct ctcgggcccg gccggggcgg gcgggggcgg cggtgggggc
     2101 gggggcggcg gcggcggcgg gggcaccaag gccaaccagg atcgggtcaa gcggcccatg
     2161 aacgccttca tggtgtggtc ccgcggacag cggcgcaaga tggcccagga aaaccccaag
     2221 atgcacaact cggagatcag caagcgcctc ggggccgagt ggaaggtcat gtccgaggcc
     2281 gagaagcggc cgttcatcga cgaggccaag agactgcgcg cgctgcacat gaaggaacac
     2341 ccggattaca agtaccggcc gcgccgcaag accaagacgc tgctcaagaa ggacaagtac
     2401 tcgctggccg gcgggctgct agcggccggc gcgggtggcg gcggcgcggc cgtggccatg
     2461 ggtgtgggcg tgggcgtcgg ggcggcggcg gtgggccagc gcttggagag cccaggcggc
     2521 gcggcgggag gaggctacgc gcatgtcaac ggctgggcta acggcgccta ccccggctcg
     2581 gtggccgcgg cggctgcggc cgcggccatg atgcaggagg cacagctggc ctacgggcag
     2641 cacccaggcg cgggcggcgc gcacccgcac gcacacccgg cgcacccgca cccgcaccat
     2701 ccgcacgcgc atcctcacaa cccgcagccc atgcaccgct acgacatggg cgcgctgcag
     2761 tacagcccca tctccaactc tcagggctac atgagcgcgt cgccttcggg ctacggcggc
     2821 atcccttacg gcgccgcggc cgccgccgcc gccgctgcgg gcggcgcgca ccagaactcg
     2881 gcggtggcgg cagcggcagc cgcggcagcc gcgtcgtcgg gggccctggg cgccctcgga
     2941 tctctggtca agtcggagcc gagcggcagt ccgccggccc cggctcactc acgggcaccg
     3001 tgtcccgggg acctgcgcga gatgatcagc atgtacctgc ccgccggcga gggtggcgac
     3061 ccggcggcgg cagcggctgc ggcggcccaa agccggctgc actcgctgcc acagcactac
     3121 cagggcgcgg gcgcgggcgt caacggcacg gtgcccctga cgcacatcta gaagggtggg
     3181 cgcgccgacc cagctttctt gtacaaagtg gtgatgagcg gccgccagca cagtggtcga
     3241 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     3301 gtaggtttgg caagctagct taagtaacgc cattttggaa ggcatggaaa aatacataac
     3361 tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa
     3421 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag
     3481 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     3541 gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt
     3601 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
     3661 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
     3721 ccctcactcg gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa
     3781 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
     3841 tgagtgattg
//
