LOCUS       sequence-78384-e        6016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression of mouse Sox3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Nakagawa M, Koyanagi M, Tanabe K, Takahashi K, Ichisaka T, Aoi T, 
            Okita K, Mochiduki Y, Takizawa N, Yamanaka S
  TITLE     Generation of induced pluripotent stem cells without Myc from mouse 
            and human fibroblasts.
  JOURNAL   Nat Biotechnol. 2008 Jan;26(1):101-6. Epub 2007 Nov 30.
  PUBMED    18059259
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol."; date: "2008-01"; volume: "26(1)"; pages: "101-6. Epub 
            2007 Nov 30"
COMMENT     Sequence Label: pMXs-ms-Sox3
FEATURES             Location/Qualifiers
     source          1..6016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1100..1516
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1446..1468
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1484..1500
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1526..1900
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1838..1854
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1951..1975
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3365..3389)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             3461..4054
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4120..4137)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4291..4879)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4371..4390)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5043..5834,5835..5903))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5666..5685
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5904..6008)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaag cggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggatctcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct tagaggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat atgggcccgg gccagactgt taccactccc ttaagtttga ccttaggtca
     1141 ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg
     1201 ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac
     1261 ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg
     1321 acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc
     1381 ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc
     1441 tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac
     1501 tccttctcta ggcgccccca tatggccata tgagatctta tatggggcac ccccgcccct
     1561 tgtaaacttc cctgaccctg acatgacaag agttactaac agcccctctc tccaagctca
     1621 cttacaggct ctctacttag tccagcacga agtctggaga cctctggcgg cagcctacca
     1681 agaacaactg gaccgaccgg tggtacctca cccttaccga gtcggcgaca cagtgtgggt
     1741 ccgccgacac cagactaaga acctagaacc tcgctggaaa ggaccttaca cagtcctgct
     1801 gaccaccccc accgccctca aagtagacgg catcgcagct tggatacacg ccgcccacgt
     1861 gaaggctgcc gaccccgggg gtggaccatc ctctagactg ccggatctag ctagttaatt
     1921 aaggatccca gtgtggtggt acgggaaatc acaagtttgt acaaaaaagc aggctccgcg
     1981 gccgccccct tcaccatgcg accagctcga gagaacgcat caggtgagag aagcccgcga
     2041 gttcccgccg actttgcgcg gagcccttcg gctagcctgc ccttcccgcc tgagctgccg
     2101 gcccgccggc ccctgagcac cactccgacg gagtcccccg gccttttcac ggtggccgct
     2161 ccagcccccg gagcgccgtc tcctcccgcc acgctggcgc acctccttcc cgccccggcg
     2221 atgtacagcc tgctggagac tgaactcaag aaccccgtgg ggccgcccac cccagccgcg
     2281 ggcaccggcg tccccgcagc tcccggcgct gcaggcaaga gtggcgcgaa cccagccggc
     2341 ggagcgaacg caggcaacgg gggcagcggg ggcgcgaacg gcggcggtgg tggtggtggc
     2401 ggcgggggca gcgaccagga ccgcgtcaag cgacccatga acgcgttcat ggtgtggtcc
     2461 cgcgggcagc ggcgcaagat ggccctggag aaccccaaga tgcacaactc cgagatcagc
     2521 aagcgcttgg gcgccgactg gaaactgctg accgatgcgg agaagcggcc gttcatcgac
     2581 gaggccaagc gactgcgtgc ggtgcacatg aaggagtacc cggactacaa gtaccggccc
     2641 cgccgcaaga ccaagacgct gctcaagaag gacaagtact cgctgcccgg cggcctcctg
     2701 cccccgggcg ccgccgcagc cgccgccgct gccgccgcag ccgccgccgc cagcagcccg
     2761 gtgggcgtgg gccagcgcct ggacacgtac acgcacgtga acggctgggc caacggcgcc
     2821 tactcgctcg tgcaggagca gctgggctac gcgcagcccc cgagcatgag cagcccgccg
     2881 ccgccacccg cgctgcctca gatgcaccgc tacgacatgg ccggcctgca gtacagcccc
     2941 atgatgccac ccggcgccca gagctacatg aacgccgccg ccgccgctgc cgccgcctcg
     3001 ggctacgggg gcatggcgcc ctccgccgcg gccgccgccg ccgccgccta cgggcagcag
     3061 cccgccaccg ctgccgccgc ggccgccgcc gccgccgcca tgagcctggg ccccatgggc
     3121 tccgtggtga agtcggagcc cagctctccg ccgcccgcca tcgcttcgca ctcgcagcgc
     3181 gcgtgcctcg gcgacctgcg cgacatgatc agcatgtacc tgccacctgg cggggacgcg
     3241 gccgacgccg cttctccgct cccaggcggc cggctgcacg gcgtgcacca gcactaccag
     3301 ggcgccggga ctgcggtcaa tggaacggtg ccgctgaccc acatctgaaa gggtgggcgc
     3361 gccgacccag ctttcttgta caaagtggtg atgagcggcc gccagcacag tggtcgacga
     3421 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     3481 ggtttggcaa gctagcttaa gtaacgccat tttggaaggc atggaaaaat acataactga
     3541 gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca
     3601 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg
     3661 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     3721 cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc
     3781 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
     3841 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
     3901 tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa
     3961 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
     4021 gtgattgact acccgtcagc gggggtcttt cattctgcat taatgaatcg gccaacgcgc
     4081 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     4141 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     4201 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4261 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     4321 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     4381 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     4441 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4501 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4561 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4621 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4681 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     4741 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4801 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4861 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4921 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4981 gatccttttg cggccggccg caaatcaatc taaagtatat atgagtaaac ttggtctgac
     5041 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5101 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5161 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5221 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5281 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5341 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5401 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5461 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5521 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5581 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5641 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     5701 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5761 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5821 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5881 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     5941 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6001 gttccgcgca catttc
//
