LOCUS       sequence-78390-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Castillon GA, Michon L, Watanabe R
  TITLE     Apical sorting of lysoGPI-anchored proteins occurs independent of 
            association with detergent-resistant membranes but dependent on 
            their N-glycosylation.
  JOURNAL   Mol Biol Cell. 2013 Jun;24(12):2021-33. doi: 
            10.1091/mbc.E13-03-0160. Epub 2013 Apr 24.
  PUBMED    23615438
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-03-0160"; journalName: "Mol Biol Cell"; date: 
            "2013-06"; volume: "24"; issue: "12"; pages: "2021-33"
COMMENT     Sequence Label: pME-puro-SNAP-FLAG-CD59
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(260..283)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(293..832)
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="KDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEVP
                     APAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVV
                     KFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAVK
                     EWLLAHEGHRLGKPGLG"
ORIGIN
        1 ctgttttctc tgataaggat gtcccaccat tctcgagctg ttcgttaaag ttacacaggt
       61 ccttcttgca gcagtagtac gttagctcat tttccctcaa gcgggttgtg acgtcgttga
      121 aattgcaatg ctcaaacttc caacacttgt tatacacttg taacccagct ttggtaatga
      181 gacacgcatc aaaatcagat gaacaattga cggctgtttt gcagtcagca gttgggttag
      241 gacagttgta gcactgaagc ttgtcatcgt cgtccttgta gtcctgcagg gcacccagcc
      301 caggcttgcc cagtctgtgg ccctcgtggg ccagcagcca ctctttcacg gcgagcccgc
      361 cctcgtagcc ccccacgtcc aggtcgccct gcaccacccg gtggcagggg atcagaatgg
      421 gcacgggatt tccgctcagg gcggttttca cggcggcggt ggcggcggga ttgccggcca
      481 gggcggccag gtggctgtag ctgatgacct ctccgaactt caccactttc agcagtttcc
      541 acagcacctg gcgggtaaag ctctcctgct ggaacactgg gtggtgcagg gctggcacag
      601 ggaactcctc gatggcctca ggctggtgaa agtaggcgtt gagccaggcg gtggcctgca
      661 tcagtggctc tggtccgccc agcacggcgg ctggggcagg cacttccacg gcgtcggcgg
      721 cagatgttcc tttgcccagg aagatgatac ggtgcaggcc ctgttcgcac ccagacagtt
      781 ccagcttgcc cagagggcta tccagggtgg tgcgcttcat ttcgcagtct tt
//
