LOCUS       sequence-78392-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of mouse Trific fused to GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Short KM, Cox TC
  TITLE     Subclassification of the RBCC/TRIM superfamily reveals a novel motif
            necessary for microtubule binding.
  JOURNAL   J Biol Chem. 2006 Mar 31;281(13):8970-80. Epub 2006 Jan 23.
  PUBMED    16434393
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2006-03-31"; volume: "281(13)"; pages: "8970-80. Epub
            2006 Jan 23"
COMMENT     Sequence Label: pcDNA-DEST53-mTrific
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(92..94,95..97,98..808)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(125..153)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     748..768
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagacac catggccagc aaaggagaag aacttttcac
      121 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      181 cagtggagag ggtgaaggtg atgctacata cggaaagctt acccttaaat ttatttgcac
      241 tactggaaaa ctacctgttc catggccaac acttgtcact actttctctt atggtgttca
      301 atgcttttcc cgttatccgg atcatatgaa acggcatgac tttttcaaga gtgccatgcc
      361 cgaaggttat gtacaggaac gcactatatc tttcaaagat gacgggaact acaagacgcg
      421 tgctgaagtc aagtttgaag gtgataccct tgttaatcgt atcgagttaa aaggtattga
      481 ttttaaagaa gatggaaaca ttctcggaca caaactcgag tacaactata actcacacaa
      541 tgtatacatc acggcagaca aacaaaagaa tggaatcaaa gctaacttca aaattcgcca
      601 caacattgaa gatggatccg ttcaactagc agaccattat caacaaaata ctccaattgg
      661 cgatggccct gtccttttac cagacaacca ttacctgtcg acacaatctg ccctttcgaa
      721 agatcccaac gaaaagcgtg accacatggt ccttcttgag tttgtaactg ctgctgggat
      781 tacacatggc atggatgagc tctacaaaag cggttccgga ccggatcaaa caagtttgta
      841 caaaaaa
//
