LOCUS       sequence-78394-e        5794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression of mouse Klf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Nakagawa M, Koyanagi M, Tanabe K, Takahashi K, Ichisaka T, Aoi T, 
            Okita K, Mochiduki Y, Takizawa N, Yamanaka S
  TITLE     Generation of induced pluripotent stem cells without Myc from mouse 
            and human fibroblasts.
  JOURNAL   Nat Biotechnol. 2008 Jan;26(1):101-6. Epub 2007 Nov 30.
  PUBMED    18059259
REFERENCE   2  (bases 1 to 5794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol."; date: "2008-01"; volume: "26(1)"; pages: "101-6. Epub 
            2007 Nov 30"
COMMENT     Sequence Label: pMXs-ms-Klf1
FEATURES             Location/Qualifiers
     source          1..5794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1100..1516
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1446..1468
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1484..1500
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1526..1900
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1838..1854
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1951..1975
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3143..3167)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             3239..3832
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3898..3915)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4069..4657)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4149..4168)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4821..5612,5613..5681))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5444..5463
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5682..5786)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaag cggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggatctcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct tagaggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat atgggcccgg gccagactgt taccactccc ttaagtttga ccttaggtca
     1141 ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg
     1201 ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac
     1261 ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg
     1321 acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc
     1381 ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc
     1441 tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac
     1501 tccttctcta ggcgccccca tatggccata tgagatctta tatggggcac ccccgcccct
     1561 tgtaaacttc cctgaccctg acatgacaag agttactaac agcccctctc tccaagctca
     1621 cttacaggct ctctacttag tccagcacga agtctggaga cctctggcgg cagcctacca
     1681 agaacaactg gaccgaccgg tggtacctca cccttaccga gtcggcgaca cagtgtgggt
     1741 ccgccgacac cagactaaga acctagaacc tcgctggaaa ggaccttaca cagtcctgct
     1801 gaccaccccc accgccctca aagtagacgg catcgcagct tggatacacg ccgcccacgt
     1861 gaaggctgcc gaccccgggg gtggaccatc ctctagactg ccggatctag ctagttaatt
     1921 aaggatccca gtgtggtggt acgggaaatc acaagtttgt acaaaaaagc aggctccgcg
     1981 gccgccccct tcaccatgag gcagaagaga gagaggaggc ctgaggtcca gggtggacac
     2041 cagccagcca tggcctcagc tgagactgtc ttaccctcca tcagtacact caccaccctg
     2101 ggacagttcc tggacaccca ggaggacttc ctcaagtggt ggcggtctga ggagacgcag
     2161 gatttggggc cggggccccc gaatcccacg gggccgtccc atcacgtgag tctgaaatcg
     2221 gaggaccctt ccggagagga cgatgagagg gacgtgacct gtgcgtggga cccggatctt
     2281 ttccttacaa actttccagg ttccgagtct cccggcactt cccggacctg tgccctggcg
     2341 cccagcgtgg ggccagtggc acagttcgag ccgcctgagt ctctgggcgc ctatgcgggt
     2401 ggcccagggt tggtgactgg gcctttgggc tccgaggagc acacaagctg ggcgcacccg
     2461 actccgagac ccccagcccc tgaacccttc gtggcccctg ccctggcccc gggactcgct
     2521 cccaaggctc agccctcgta ctccgactcg cgagcgggct ccgtaggggg cttcttcccg
     2581 cgggcggggc ttgcggtgcc cgcagctcca ggcgccccct atgggctgct gtcgggatac
     2641 cccgcgctgt accccgcgcc acagtaccaa ggccacttcc agctctttcg cgggctcgcg
     2701 gcgccttctg ctggtcccac ggcgccccct tccttcttga attgtctggg acctgggact
     2761 gtggccacag aactcggggc cactgcgatc gccggagacg caggcttgtc cccgggaact
     2821 gcgccgccca aacgcagccg gcgaactttg gcacctaaga ggcaggcggc acatacgtgc
     2881 gggcacgaag gctgcgggaa gagctacacc aagagctcgc acctcaaggc gcacctgcgc
     2941 acgcacacgg gagagaagcc ttatgcctgc tcctgggacg gctgtgactg gaggttcgct
     3001 cgctcagacg aactgacgcg ccactaccgg aagcacactg gacatcgtcc cttctgctgt
     3061 ggcctctgcc cacgtgcttt ttcacgctct gaccacttag ctctgcacat gaagcgtcac
     3121 ctctgaaagg gtgggcgcgc cgacccagct ttcttgtaca aagtggtgat gagcggccgc
     3181 cagcacagtg gtcgacgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     3241 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tggaaggcat
     3301 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag
     3361 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     3421 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     3481 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
     3541 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     3601 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     3661 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
     3721 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     3781 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca ttctgcatta
     3841 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     3901 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     3961 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     4021 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4081 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4141 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4201 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4261 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4321 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4381 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4441 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4501 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4561 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4621 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4681 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     4741 aaaaaggatc ttcacctaga tccttttgcg gccggccgca aatcaatcta aagtatatat
     4801 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4861 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4921 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4981 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     5041 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     5101 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     5161 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     5221 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     5281 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     5341 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     5401 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     5461 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     5521 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     5581 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     5641 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     5701 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     5761 aaaaataaac aaataggggt tccgcgcaca tttc
//
