LOCUS       sequence-78395-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Castillon GA, Michon L, Watanabe R
  TITLE     Apical sorting of lysoGPI-anchored proteins occurs independent of 
            association with detergent-resistant membranes but dependent on 
            their N-glycosylation.
  JOURNAL   Mol Biol Cell. 2013 Jun;24(12):2021-33. doi: 
            10.1091/mbc.E13-03-0160. Epub 2013 Apr 24.
  PUBMED    23615438
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-03-0160"; journalName: "Mol Biol Cell"; date: 
            "2013-06"; volume: "24"; issue: "12"; pages: "2021-33"
COMMENT     Sequence Label: pME-puro-SNAP-FLAG-CD59
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             93..638
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             648..671
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 attccaccat gggaatccaa ggagggtctg tcctgttcgg gctgctgctc gtcctggctg
       61 tcttctgcca ttcaggtcat agcctgcagg gcatggacaa agactgcgaa atgaagcgca
      121 ccaccctgga tagccctctg ggcaagctgg aactgtctgg gtgcgaacag ggcctgcacc
      181 gtatcatctt cctgggcaaa ggaacatctg ccgccgacgc cgtggaagtg cctgccccag
      241 ccgccgtgct gggcggacca gagccactga tgcaggccac cgcctggctc aacgcctact
      301 ttcaccagcc tgaggccatc gaggagttcc ctgtgccagc cctgcaccac ccagtgttcc
      361 agcaggagag ctttacccgc caggtgctgt ggaaactgct gaaagtggtg aagttcggag
      421 aggtcatcag ctacagccac ctggccgccc tggccggcaa tcccgccgcc accgccgccg
      481 tgaaaaccgc cctgagcgga aatcccgtgc ccattctgat cccctgccac cgggtggtgc
      541 agggcgacct ggacgtgggg ggctacgagg gcgggctcgc cgtgaaagag tggctgctgg
      601 cccacgaggg ccacagactg ggcaagcctg ggctgggtgc cctgcaggac tacaaggacg
      661 acgatgacaa gcttcagtgc tacaactgtc ctaacccaac tgctgactgc aaaacagccg
      721 tcaattgttc atctgatttt gatgcgtgtc tcattaccaa agctgggtta caagtgtata
      781 acaagtgttg gaagtttgag cattgcaatt tcaacgacgt cacaacccgc ttgagggaaa
      841 atgagctaac gtactactgc tgcaagaagg acctgtgtaa ctttaacgaa cagctcgaga
      901 atggtg
//
