LOCUS       sequence-78401-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Castillon GA, Michon L, Watanabe R
  TITLE     Apical sorting of lysoGPI-anchored proteins occurs independent of 
            association with detergent-resistant membranes but dependent on 
            their N-glycosylation.
  JOURNAL   Mol Biol Cell. 2013 Jun;24(12):2021-33. doi: 
            10.1091/mbc.E13-03-0160. Epub 2013 Apr 24.
  PUBMED    23615438
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E13-03-0160"; journalName: "Mol Biol Cell"; date: 
            "2013-06"; volume: "24"; issue: "12"; pages: "2021-33"
COMMENT     Sequence Label: pME-puro-CLIP-FLAG-CD59
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(400..423)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttctctag actagtctag cggccgctta gggatgaagg ctccaggctg ctgccagaaa
      121 tggagtcacc agcagaagaa ctgttttctc tgataaggat gtcccaccat tctcgagctg
      181 ttcgttaaag ttacacaggt ccttcttgca gcagtagtac gttagctcat tttccctcaa
      241 gcgggttgtg acgtcgttga aattgcaatg ctcaaacttc caacacttgt tatacacttg
      301 taacccagct ttggtaatga gacacgcatc aaaatcagat gaacaattga cggctgtttt
      361 gcagtcagca gttgggttag gacagttgta gcactgaagc ttgtcatcgt cgtccttgta
      421 gtcctgcagg gcacccagcc caggcttgcc cagtctgtgg ccctcgtggg ccagcagcca
      481 ctctttcacg gcgagcccgc ccaggtaggg ccccacgtcg ctgtcgccct gcaccacccg
      541 gtggcagggg atcagaatgg gcacgggatt tccgtccagg gcggtgttca cggcggcggt
      601 ggcggcggga ttgcccacca gggcggccag gtggctctcg ctgatgacct ctccgaactt
      661 caccactttc agcagtttcc acagcacctg gcgggtaaag ctctcctgct ggaacactgg
      721 gtggtgcagg gctggcacag ggaactcctc gatggcctca ggctggtgaa agtaggcgtt
      781 gagccaggcg gtggcctgga tcagtggctc tggtccgccc agcacggcgg ctggggcagg
      841 cacttccacg gcgtc
//
