LOCUS       sequence-78409-e        1123 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTAL plasmid compatible with the Golden Gate TALEN cloning system, 
            for cloning of TALENs and expression in mammalian cells. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1123)
  TITLE     TALEN Golden Gate vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTALEN(Nd154C63) MND
FEATURES             Location/Qualifiers
     source          1..1123
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    745..766
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(781..786,787..804,805..811)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    819..835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     824..846
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     843..859
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     843..859
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             855..1121
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLN"
     primer_bind     878..898
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        880..898
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(945..964)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(946..964)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(974..991)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(974..990)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(983..1005)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cttcctcaag tgtcaatgta ccagctttaa tcatttctcc accaatcaaa agctcttcta
       61 cactaagaac agctccatta caattagtga tatgattcaa tcttgtaagc tgagctttgt
      121 agtttccttt gaaatgacca ctcacaaaca agaacttaaa ttctgttact gaagatggat
      181 agactttcca ccattcatta gggttgatat gcttgtttct tgtttgattc tcttcgacat
      241 atctttgcat ttcatctgct tgaccaattg gaagattata acctcctgaa tatgccttag
      301 tatcaacgat cacaccgtaa tcaataggag atccgacagt ataaattgct ccgtctggtt
      361 tccttgatcc acccaaatgt ttaccacgat aaccataaac cttcataaag aactccatca
      421 ccttcatttc aaggattcta tcctgagttg aatttcttgc gatttcaatc aattcaatat
      481 attcatgtgg cacatatttc aatttatgtc taagttcaga tttcttctct tccaattcag
      541 atttcactag ctgggatcta gatatcgcaa cgcgatggga cgtgcgttcg ccaatacggc
      601 gattgactct tctgatcaat tccggcgcgt gcggcaatcc ctttttcact gcatccatgg
      661 caggacgtcc gccgaggcag gccaaggcga cgaggtggtc gttggagacg accggtggaa
      721 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
      781 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
      841 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
      901 acaaaagctg gaggacctcg agggggggcc cggtacccaa ttcgccctat agtgagtcgt
      961 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     1021 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     1081 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tgg
//
