LOCUS       sequence-78416-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SSR1.0 has a one I-SceI target site for generate specific double 
            strand break after I-SceI endonuclease expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Gomez-Cabello D, Jimeno S, Fernandez-Avila MJ, Huertas P
  TITLE     New tools to study DNA double-strand break repair pathway choice.
  JOURNAL   PLoS One. 2013 Oct 14;8(10):e77206. doi: 
            10.1371/journal.pone.0077206.
  PUBMED    24155929
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-14"; volume: "8(10):e77206. doi"; pages: " 
            10.1371/journal.pone.0077206"
COMMENT     Sequence Label: SeeSaw Reporter (SSR1.0)
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    92..680
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(145..165)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(563..574)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggacgaagct gtacaagtcc ggactcagat ctcgagctca agcttcgaat tgtagggata
       61 acagggtaat cgaattctgc agtcgatcga caatcaacct ctggattaca aaatttgtga
      121 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
      181 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
      241 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
      301 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
      361 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
      421 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
      481 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
      541 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
      601 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
      661 cctttgggcc gcctccccgc ctggaattcg agctcgtcga cacccagagc atgagaatcc
      721 acgtgaccga gggcgccccc ctgcccttcg ccttcgacat cctggccccc tgctgcgagt
      781 acggcagcag gaccttcgtg caccacaccg ccgagatccc cgacttcttc aagcagagct
      841 tccccgaggg cttcacctgg gagagaacca ccacctac
//
