LOCUS       sequence-78427-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Venus and FLAG-tagged human CD59, a GPI-AP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Rivier AS, Castillon GA, Michon L, Fukasawa M, Romanova-Michaelides 
            M, Jaensch N, Hanada K, Watanabe R
  TITLE     Exit of GPI-anchored proteins from the ER differs in yeast and 
            mammalian cells.
  JOURNAL   Traffic. 2010 Aug;11(8):1017-33. doi: 
            10.1111/j.1600-0854.2010.01081.x. Epub 2010 May 11.
  PUBMED    20477992
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2010.01081.x"; journalName: "Traffic"; date: 
            "2010-08"; volume: "11"; issue: "8"; pages: "1017-33"
COMMENT     Sequence Label: pME-puro-Venus-FLAG-CD59
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(127..155)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     750..770
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gctgcggatt ccaccatggg aatccaagga gggtctgtcc tgttcgggct gctgctcgtc
       61 ctggctgtct tctgccattc aggtcatagc ctgcagggca gtaaaggaga agaacttttc
      121 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      181 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa attgatttgc
      241 actactggaa aactacctgt tccatggcca acacttgtca ctactttggg ttatggtcta
      301 atgtgctttg ctagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg
      361 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
      421 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
      481 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
      541 aatgtataca tcactgcaga caaacaaaag aatggaatca aagctaactt caaaattaga
      601 cacaacattg aagatggagg tgttcaacta gcagaccatt atcaacaaaa tactccaatt
      661 ggcgatggcc ctgtcctttt accagacaac cattacctgt cctatcaatc tgccctttcg
      721 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
      781 attacacatg gcatggatga actatacaaa gccctgc
//
