LOCUS       sequence-78428-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express mCherry and FLAG-tagged human CD59, a GPI-AP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Rivier AS, Castillon GA, Michon L, Fukasawa M, Romanova-Michaelides 
            M, Jaensch N, Hanada K, Watanabe R
  TITLE     Exit of GPI-anchored proteins from the ER differs in yeast and 
            mammalian cells.
  JOURNAL   Traffic. 2010 Aug;11(8):1017-33. doi: 
            10.1111/j.1600-0854.2010.01081.x. Epub 2010 May 11.
  PUBMED    20477992
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2010.01081.x"; journalName: "Traffic"; date: 
            "2010-08"; volume: "11"; issue: "8"; pages: "1017-33"
COMMENT     Sequence Label: pME-mCherry-FLAG-CD59-GPI
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(376..399)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(681..700)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cagggggagg tgtgggaggt tttttctcta gactagtcta gcggccgctt acagagaaat
       61 gaagtccagg gcttggagga gggagaggga aagcagcagc agcagcatcc aggatgtgtt
      121 ctgaaccagc aggcttatgc cgccattctc gagctgttcg ttaaagttac acaggtcctt
      181 cttgcagcag tagtacgtta gctcattttc cctcaagcgg gttgtgacgt cgttgaaatt
      241 gcaatgctca aacttccaac acttgttata cacttgtaac ccagctttgg taatgagaca
      301 cgcatcaaaa tcagatgaac aattgacggc tgttttgcag tcagcagttg ggttaggaca
      361 gttgtagcac tgaagcttgt catcgtcgtc cttgtagtcc tgcatcttgt acagctcgtc
      421 catgccgccg gtggagtggc ggccctcggc gcgttcgtac tgttccacga tggtgtagtc
      481 ctcgttgtgg gaggtgatgt ccaacttgat gttgacgttg taggcgccgg gcagctgcac
      541 gggcttcttg gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag
      601 cttcagcctc tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga
      661 ggaggcctcc cagcccatgg tcttcttctg cattacgggg ccgtcggagg ggaagttggt
      721 gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc agggaggagt cctgggtcac
      781 ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggg
//
