LOCUS       sequence-78429-e         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express mCherry and FLAG-tagged human CD59, a GPI-AP in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Rivier AS, Castillon GA, Michon L, Fukasawa M, Romanova-Michaelides 
            M, Jaensch N, Hanada K, Watanabe R
  TITLE     Exit of GPI-anchored proteins from the ER differs in yeast and 
            mammalian cells.
  JOURNAL   Traffic. 2010 Aug;11(8):1017-33. doi: 
            10.1111/j.1600-0854.2010.01081.x. Epub 2010 May 11.
  PUBMED    20477992
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1600-0854.2010.01081.x"; journalName: "Traffic"; date: 
            "2010-08"; volume: "11"; issue: "8"; pages: "1017-33"
COMMENT     Sequence Label: pME-mCherry-FLAG-CD59-GPI
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(240..258)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 agctgcggat tccaccatgg gaatccaagg agggtctgtc ctgttcgggc tgctgctcgt
       61 cctggctgtc ttctgccatt caggtcatag cctgcatgtg agcaagggcg aggaggataa
      121 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      181 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      241 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      301 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      361 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      421 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      481 gcgcggcacc aacttcccct ccgacggccc cgtaatgcaa aagaagacca tgggctggga
      541 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
      601 gctgaagctg aaggacggcg gccactacga cgctga
//
